About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:223046 Rieusset A, et al., Stochastic loss of silencing of the imprinted Ndn/NDN allele, in a mouse model and humans with prader-willi syndrome, has functional consequences. PLoS Genet. 2013;9(9):e1003752
Assay type: RT-PCR
MGI Accession ID: MGI:6450168
Gene symbol: Ndn
Gene name: necdin, MAGE family member
Probe: Ndn-pA, Ndn-pB
Assay notes: Quantitative RT-PCR. For each sample, the number of Ndn cDNA copies was normalized according to relative efficiency of RT determined by the standard cDNA quantification.
Results
Image: 1G ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
F E12 E12.5 TS20: embryo Present (a) 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Female
M E12 E12.5 TS20: embryo Present 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Male
F P1 Brain P1 TS27: brain Present (a) 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Female
M P1 Brain P1 TS27: brain Present 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Male
F P1 Hypoth P1 TS27: hypothalamus Present (a) 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Female
M P1 Hypoth P1 TS27: hypothalamus Present 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Male
F P1 Pons P1 TS27: pons Present (a) 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Female
M P1 Pons P1 TS27: pons Present 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Male
Notes:
(a) Female mice have a 2-fold increase in expression compared to males.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory