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Gene Expression Data
Assay Details
Assay
Reference: J:223046 Rieusset A, et al., Stochastic loss of silencing of the imprinted Ndn/NDN allele, in a mouse model and humans with prader-willi syndrome, has functional consequences. PLoS Genet. 2013;9(9):e1003752
Assay type: RT-PCR
MGI Accession ID: MGI:6450167
Gene symbol: Ndn
Gene name: necdin, MAGE family member
Probe: Ndn-pA, Ndn-pB
Assay notes: Quantitative RT-PCR. For each sample, the number of Ndn cDNA copies was normalized according to relative efficiency of RT determined by the standard cDNA quantification.
Results
Image: 1F ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
F wt x M -m/+p P1 TS27: pons Present (a) 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Not Specified
F wt x M +m/-p P1 TS27: pons Present (a) 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Not Specified
F -m/+p x M -/- P1 TS27: pons Present (b) 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Not Specified
F +m/-p x M -/- P1 TS27: pons Present (c) 1 µg; total RNA involves: 129S2/SvPas * C57BL/6J Ndntm1.1Mus/Ndn+Not Specified
Notes:
(a) The paternal genotype did not have a significant effect on expression.
(b) Embryos from Ndn2m/+p mothers had a lower level of expression.
(c) Embryos from the Ndn+/+ or Ndn+m/2p maternal genotype had a three-fold higher level of Ndn maternal expression compared to those from a Ndn2m/+p maternal genotype.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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