Ndn RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:223046 Rieusset A, et al., Stochastic loss of silencing of the imprinted Ndn/NDN allele, in a mouse model and humans with prader-willi syndrome, has functional consequences. PLoS Genet. 2013;9(9):e1003752
Assay type:	RT-PCR
MGI Accession ID:	MGI:6450166
Gene symbol:	Ndn
Gene name:	necdin, MAGE family member

Probe:	Ndn-pA, Ndn-pB
Assay notes:	Quantitative RT-PCR. For each sample, the number of Ndn cDNA copies was normalized according to relative efficiency of RT determined by the standard cDNA quantification.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
F wt x M -m/+p	P1	TS27: brain	Present (a)	1 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Ndn^tm1.1Mus/Ndn⁺	Not Specified
F wt x M +m/-p	P1	TS27: brain	Present (a)	1 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Ndn^tm1.1Mus/Ndn⁺	Not Specified
F -m/+p x M -/-	P1	TS27: brain	Present (b)	1 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Ndn^tm1.1Mus/Ndn⁺	Not Specified
F +m/-p x M -/-	P1	TS27: brain	Present (c)	1 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Ndn^tm1.1Mus/Ndn⁺	Not Specified

Notes:
(a) The paternal genotype did not have a significant effect on expression.
(b) Embryos from Ndn2m/+p mothers had a lower level of expression.
(c) Embryos from the Ndn+/+ or Ndn+m/2p maternal genotype had a three-fold higher level of Ndn maternal expression compared to those from a Ndn2m/+p maternal genotype.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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