Probe:	cypher cDNA5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The probe used in this assay is for the carboxyl terminus of the longer transcript; assays using a probe for the amino terminus (MGI:6449999), which recognizes both transcripts, generated identical results.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Strong		3A	No expression was detected outside the myocardium.
TS15: endocardial lining	Absent		3A

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Strong		3B	Non-cardiac rexpression was undetectable.
TS20: ventricle cardiac muscle	Strong		3B	Non-cardiac rexpression was undetectable.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle tissue	Weak			Non-cardiac striated muscle begins to show expression at this time.
TS20: cardiac muscle tissue	Strong

Structure	Level	Pattern	Image	Note
TS22: intercostal muscle	Present		4A
TS22: skeletal muscle	Present		4A

Structure	Level	Pattern	Image	Note
TS22: atrium cardiac muscle	Strong		4B
TS22: diaphragm	Strong		4B
TS22: ventricle cardiac muscle	Strong		4B
TS22: pulmonary trunk	Present		4B
TS22: cartilage condensation	Absent		4B
TS22: endocardial lining	Absent		4B

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Strong		4C
TS22: intercostal muscle	Present		4C
TS22: liver	Absent		4C

Structure	Level	Pattern	Image	Note
TS22: abdomen musculature	Strong
TS22: limb muscle	Strong
TS22: tongue muscle	Strong