Gene Expression Data
Assay Details
Assay
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Reference:
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J:175550
Valasek P, et al., Cellular and molecular investigations into the development of the pectoral girdle. Dev Biol. 2011 Sep 1;357(1):108-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6449208
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Gene symbol:
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Myod1
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Gene name:
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myogenic differentiation 1
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Probe:
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Myod1 cDNA8
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1E:
embryonic day 10.5
(more )
Specimen
1E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk mesenchyme |
Present |
Regionally restricted |
1E
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Expression is in the ventral pre-muscle mass onto the thorax forming pectoral muscles.
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Specimen
1E':
embryonic day 10.5
(more )
Specimen
1E':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk mesenchyme |
Present |
Regionally restricted |
1E'
|
Expression is in the ventral pre-muscle mass onto the thorax forming pectoral muscles.
|
Specimen
1F:
embryonic day 11.0
(more )
Specimen
1F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: trunk mesenchyme |
Present |
Regionally restricted |
1F
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Expression is in the ventral pre-muscle mass onto the thorax forming pectoral muscles, the diaphragm, and the intercostal muscles.
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Specimen
1F':
embryonic day 11.0
(more )
Specimen
1F':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: trunk mesenchyme |
Present |
Regionally restricted |
1F'
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Expression is in the latissimus dorsi.
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Specimen
1G:
embryonic day 12.0
(more )
Specimen
1G:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: skeletal muscle tissue |
Present |
Regionally restricted |
1G
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Expression is in the ventral pre-muscle mass onto the thorax forming pectoral muscles.
|
TS20: trunk musculature |
Present |
Regionally restricted |
1G
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Expression is in the ventral pre-muscle mass onto the thorax forming pectoral muscles.
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Specimen
1G':
embryonic day 12.0
(more )
Specimen
1G':
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
1H:
embryonic day 12.5
(more )
Specimen
1H:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: skeletal muscle tissue |
Present |
Regionally restricted |
1H
|
Expression is in the ventral pre-muscle mass onto the thorax forming pectoral muscles.
|
TS20: trunk musculature |
Present |
Regionally restricted |
1H
|
Expression is in the ventral pre-muscle mass onto the thorax forming pectoral muscles.
|
Specimen
1H':
embryonic day 12.5
(more )
Specimen
1H':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
1I:
embryonic day 13.5
(more )
Specimen
1I:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 13.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
1I':
embryonic day 13.5
(more )
Specimen
1I':
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
6A:
embryonic day 12.5
(more )
Specimen
6A:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6B:
(close )
Genetic Background:
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involves: C57BL/6J * SJL/J
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Mutant Allele(s):
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Tbx5tm1Jse/Tbx5tm1Jse Tg(Prrx1-cre)1Cjt/0
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: skeletal muscle tissue |
Present |
Regionally restricted |
6B
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This mutant has abnormal/absent pectoral girdle and diaphragm and lacks forelimbs. Expression is in the trapezius and intercostal muscles.
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