Probe:	Cbs cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: alimentary system mesenchyme	Present		2E right
TS20: liver	Present		2E right
TS20: head bone	Present	Regionally restricted	2E right	Expression was detected in chondroblasts of the developing bones of the head.
TS20: cartilage	Present	Regionally restricted	2E right	Expression was detected in pharyngolaryngeal cartilage.
TS20: rib pre-cartilage condensation	Present		2E right
TS20: vertebral cartilage condensation	Present		2E right
TS20: spinal cord	Weak		2E right

Structure	Level	Pattern	Image	Note
TS21: vertebral pre-cartilage condensation	Present	Regionally restricted	3_1A/B	Expression was detected in the vertebral bodies and arches.
TS21: intervertebral disc	Absent		3_1A/B

Structure	Level	Pattern	Image	Note
TS27: perichondrium	Present		3_1C
TS27: rib	Present	Regionally restricted	3_1C	Expression was detected in chondroblasts of ribs.

Structure	Level	Pattern	Image	Note
TS28: vertebra	Present		3_1D

Structure	Level	Pattern	Image	Note
TS28: cartilage	Present
TS28: perichondrium	Present
TS28: periosteum	Present

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Strong		4A
TS20: medulla oblongata basal plate ventricular layer	Strong		4A
TS20: 4th ventricle	Strong		4A	Expression was detected in the ventricular zone.
TS20: corpus striatum	Strong		4A	Expression was detected in the ventricular zone.
TS20: medulla oblongata	Weak		4A
TS20: midbrain	Weak		4A
TS20: neocortex	Weak		4A

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Strong		4B
TS23: medulla oblongata	Weak		4B
TS23: midbrain	Weak		4B
TS23: neocortex	Weak		4B
TS23: nervous system	Present		4B	Although the strong expression in the ventricular zone of brain persisted at E15.5, the expression in the nervous system declined in intensity.

Structure	Level	Pattern	Image	Note
TS27: brain	Weak	Uniform	4C

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		4D
TS27: main olfactory bulb	Present		4D

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		4E

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		4F
TS28: dentate gyrus	Strong		4F
TS28: hippocampus	Strong		4F
TS28: brain	Weak		4F