Probe:	Nbs1 cDNA1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: gut	Strong		2a/b
TS15: nervous system	Strong		2a/b
TS15: labyrinthine zone	Present		2a/b
TS15: yolk sac	Present		2a/b
TS15: extraembryonic component	Present	Regionally restricted	2a/b	Expression is in the membranes.
TS15: embryo	Present	Widespread	2a/b

Structure	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	Present	Regionally restricted	2c/d	Expression is in the subventricular zone.
TS20: cardiac muscle tissue	Present		2c/d
TS20: gut	Present		2c/d
TS20: liver	Present		2c/d
TS20: lung	Present		2c/d
TS20: spinal cord ventricular layer	Present		2c/d
TS20: telencephalon subventricular zone	Present		2c/d
TS20: thalamus	Present		2c/d
TS20: tongue muscle	Present		2c/d
TS20: ganglionic eminence	Present	Regionally restricted	2c/d	Expression is in the ganglionic eminence projecting into the third ventricle.
TS20: telencephalon mantle layer	Absent		2c/d

Structure	Level	Pattern	Image	Note
TS22: detrusor muscle of bladder	Strong		2e/f
TS22: intercostal muscle	Strong		2e/f
TS22: thymus primordium	Strong		2e/f
TS22: tongue muscle	Strong	Regionally restricted	2e/f	Expression is in the transverse striated muscle fibers.
TS22: atrium cardiac muscle	Present		2e/f
TS22: brain	Present		2e/f
TS22: bronchiole epithelium	Present		2e/f
TS22: bronchus epithelium	Present		2e/f
TS22: dental lamina	Present		2e/f
TS22: gut	Present		2e/f
TS22: gut epithelium	Present		2e/f
TS22: gut muscularis	Present		2e/f
TS22: liver	Present		2e/f
TS22: lower jaw mesenchyme	Present		2e/f
TS22: metanephros	Present		2e/f
TS22: upper jaw mesenchyme	Present		2e/f
TS22: ventricle cardiac muscle	Present		2e/f
TS22: bronchiole	Present	Regionally restricted	2e/f	Expression is in the mesenchyme surrounding the epithelium.
TS22: bronchus mesenchyme	Present	Regionally restricted	2e/f	Expression is in the mesenchyme surrounding the epithelium.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		3a/b
TS22: inferior colliculus	Present		3a/b
TS22: medulla oblongata	Present		3a/b
TS22: midbrain tegmentum	Present		3a/b

Structure	Level	Pattern	Image	Note
TS27: cerebellum internal granule cell layer	Present		3c/d
TS27: cerebellum external granule cell layer	Present	Regionally restricted	3c/d	Expression is in the proliferative zone.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3e/f
TS28: cerebellum granule cell layer	Weak		3e/f
TS28: cerebellum molecular layer	Absent		3e/f

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Strong	Regionally restricted	3g/h	Expression is in the tubules os stages IX-XII of the gametogenic maturation cycle with lower expression in stage I-VIII tubules.
TS28: primary spermatocyte	Present	Regionally restricted	3g/h	Expression is in primary spermatocytes of the preleptotene to zygotene stage.
TS28: spermatid	Absent		3g/h
TS28: spermatogonium	Absent		3g/h
TS28: spermatozoon	Absent		3g/h

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Strong	Single cells	3i/j
TS28: spleen germinal center	Absent		3i/j

Structure	Level	Pattern	Image	Note
TS28: thymus medullary core	Strong	Single cells	3k/l

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch maxillary component	Present	Regionally restricted		Expression is in the proliferating cell populations.
TS17: forebrain	Present	Regionally restricted		Expression is in the proliferating cell populations.
TS17: future spinal cord	Present	Regionally restricted		Expression is in the proliferating cell populations.
TS17: hindbrain	Present	Regionally restricted		Expression is in the proliferating cell populations.
TS17: liver	Present	Regionally restricted		Expression is in the proliferating cell populations.
TS17: midbrain	Present	Regionally restricted		Expression is in the proliferating cell populations.

Structure	Level	Pattern	Image	Note
TS25: metanephros	Present	Regionally restricted		Expression is in the proliferative zone.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Weak
TS28: dorsal root ganglion	Absent