Probe:	pMNET3UTRA-
Probe preparation:	labelled with digoxigenin
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	2A	Expression in two distinct regions of spinal cord. Expression excluded from dorsal-most spinal cord.
TS17: neural tube floor plate	Weak		2A
TS17: neural tube lateral wall	Strong		2A	Lower level of expression by neural progenitor cells in ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Trace		2B

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Graded	2G	Graded expression in domain extending from floor plate into dorsal spinal cord.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Trace		2H
TS19: neural tube ventricular layer	Absent		2H

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Graded	2K	Bilaterally symmetric domains of graded expression in ventricular zone. Commissural axons have resolved into medial and lateral fascicles.
TS20: spinal cord lateral wall	Present	Regionally restricted	2K	Additional domain of expression in lateral region, immediately adjacent to dorsal root entry zone (DREZ).

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Ambiguous		2L
TS20: spinal cord ventricular layer	Absent		2L