Gene Expression Data
Assay Details
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Assay
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Reference:
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J:248983
Varadarajan SG, et al., Netrin1 establishes multiple boundaries for axon growth in the developing spinal cord. Dev Biol. 2017 Oct 1;430(1):177-187
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6446811
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Gene symbol:
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Ntn1
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Gene name:
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netrin 1
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Results
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Specimen
2D:
(close  )
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Genetic Background:
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involves: 129/Sv * 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
2D
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Broad expression in spinal cord. Low levels of expression in ventricular zone. Expression on pial surface at circumference of spinal cord. Expression excluded from dorsal-most spinal cord.
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129/Sv * 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
2J
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Expression on pial surface ventral to dorsal root entry zone (DREZ) as well as commissural axons.
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Specimen
2N:
(close )
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Genetic Background:
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involves: 129/Sv * 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3E:
embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1 +
(more  )
Note: Double labeled: red - Ntn1; green - neurofilament.
Specimen
3E:
(close )
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Genetic Background:
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involves: 129/Sv * 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntn1; green - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
3E
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Expression on pial surface coincident with position of ventrally-directed axons growth in mantle layer.
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Specimen
3F:
embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1 +
(more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Specimen
3F:
(close )
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Genetic Background:
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involves: 129/Sv * 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntn1; green - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
3F ntn1
,
3F
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Expression on pial surface coincident with position of ventrally-directed axons growth in mantle layer.
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Specimen
4B:
embryonic day 11.5; Ntn1Gt(ST629)Byg/Ntn1 +
(more )
Note: Triple labeled: red - Ntn1; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Specimen
4B:
(close )
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Genetic Background:
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involves: 129/Sv * 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Ntn1; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
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Specimen
4I:
embryonic day 12.5
(more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Specimen
4I:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntn1; green - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4I
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Expression on pial surface of ventral spinal cord.
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Specimen
4J:
embryonic day 12.5
(more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Specimen
4J:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntn1; green - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4J
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Expression on pial surface of dorsal spinal cord ventral to dorsal root entry zone (DREZ).
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Specimen
4K:
embryonic day 12.5
(more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Specimen
4K:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntn1; green - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4K
,
4K ntn1
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Expression on pial surface of spinal cord.
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