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Gene Expression Data
Assay Details
Assay
Reference: J:290235 Archambault D, et al., Protein phosphatase 1 regulatory subunit 35 is required for ciliogenesis, notochord morphogenesis, and cell-cycle progression during murine development. Dev Biol. 2020 Jul 3;465(1):1-10
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6446133
Gene symbol: Foxa2
Gene name: forkhead box A2
Antibody: anti-Foxa2 (M-20; sc-6554)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4I: embryonic day 9.5 (more )
Note: Littermate control; this embryo might have been a heterozygote. Transverse section; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS15: notochord Present 4I
TS15: neural tube floor plate Present 4I
Specimen 4J: embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Transverse section, most anterior of series; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS15: notochord Present 4J In the mutants, when the notochord is present, it is usually mispositioned and shifted lateral to the neural tube.
TS15: neural tube floor plate Absent 4J
Specimen 4J': embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Transverse section, part of series; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS15: notochord Present 4J' In the mutants, when the notochord is present, it is usually mispositioned and shifted lateral to the neural tube.
TS15: neural tube floor plate Absent 4J'
Specimen 4J'': embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Transverse section, part of series; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS15: notochord Present 4J'' In the mutants, when the notochord is present, it is usually mispositioned and shifted lateral to the neural tube.
TS15: neural tube floor plate Absent 4J''
Specimen 4J''': embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Transverse section, most posterior of series; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS15: notochord Absent 4J''' In the mutants, when the notochord is present, it is usually mispositioned and shifted lateral to the neural tube.
TS15: neural tube floor plate Absent 4J'''
Specimen 5A: embryonic day 8.5 (more )
Note: Littermate control; this embryo might have been a heterozygote. Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Present 5A
TS13: notochord Present 5A inset , 5A
Specimen 5A': embryonic day 8.5 (more )
Note: Littermate control; this embryo might have been a heterozygote. Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Present 5A'
TS13: notochord Present 5A' , 5A' inset
Specimen 5A'': embryonic day 8.5 (more )
Note: Littermate control; this embryo might have been a heterozygote. Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Present 5A''
TS13: notochord Present 5A'' , 5A'' inset
Specimen 5B: embryonic day 8.75; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Absent 5B
TS13: notochord Absent 5B , 5B inset Notochord resolution is altered in the mutant.
Specimen 5B': embryonic day 8.75; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Absent 5B'
TS13: notochord Absent 5B' inset , 5B' Notochord resolution is altered in the mutant.
Specimen 5B'': embryonic day 8.75; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Absent 5B''
TS13: notochord Absent 5B'' inset , 5B'' Notochord resolution is altered in the mutant.
Specimen 5C: embryonic day 8.75; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Absent 5C
TS13: notochord Present 5C , 5C inset Notochord resolution is altered in the mutant.
Specimen 5C': embryonic day 8.75; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Absent 5C'
TS13: notochord Present 5C' , 5C' inset Notochord resolution is altered in the mutant.
Specimen 5C'': embryonic day 8.75; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Consecutive sections through embryo; also stained with anti-laminin (green) to outline the basement membrane.
Structure Level Pattern Image Note
TS13: neural tube floor plate Absent 5C''
TS13: notochord Present 5C'' inset , 5C'' Notochord resolution is altered in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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