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Gene Expression Data
Assay Details
Assay
Reference: J:290606 Marcho C, et al., Tissue-specific regulation of Igf2r/Airn imprinting during gastrulation. Epigenetics Chromatin. 2015;8:10
Assay type: RT-PCR
MGI Accession ID: MGI:6444889
Gene symbol: Igf2r
Gene name: insulin-like growth factor 2 receptor
Probe: Igf2r-F,Igf2r-R
Visualized with: Ethidium bromide
Assay notes: Single-strand conformation polymorphism (SSCP) analysis was used to determine whether expression was from the maternal or paternal allele. The four embryos used in this experiment were from a single litter but ranged in developmental age from 1 (least advanced) to 4 (most advanced).
Results
Image: 3B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
1 E7.0 TS10: embryo Present Present Not Specified; total RNA (C57BL/6J x PWD/PhJ)F1 Not Specified least advanced developmental age
2 E7.0 TS10: embryo Present Present Not Specified; total RNA (C57BL/6J x PWD/PhJ)F1 Not Specified 2nd most advanced developmental age
3 E7.0 TS10: embryo Present Present Not Specified; total RNA (C57BL/6J x PWD/PhJ)F1 Not Specified 3rd most advanced developmental age
4 E7.0 TS10: embryo Present Present Not Specified; total RNA (C57BL/6J x PWD/PhJ)F1 Not Specified most advanced developmental age
B6 P adult TS28: metanephros Not Applicable Present Not Specified; total RNA C57BL/6J Not Specified
PWD P adult TS28: metanephros Present Not Applicable Not Specified; total RNA PWD/PhJ Not Specified
Notes:
(a) This band represents expression from the paternal (PWD/PhJ) allele.
(b) This band represents expression from the maternal (C57BL/6J) allele.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory