Probe:	Tcof1 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: 1st branchial arch	Very strong		6A
TS12: rhombomere	Very strong	Regionally restricted	6A	Expression is at the rhombomere boundaries.
TS12: future brain neural fold	Very strong	Regionally restricted	6A	Expression is immediately rostral to their point of fusion.
TS12: embryo	Strong		6A

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Strong		6B
TS13: future prosencephalon	Strong		6B
TS13: notochord	Strong		6B
TS13: rhombomere	Strong		6B

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Very strong	Regionally restricted	6C	Expression is at the edges of the neural folds immediately prior to fusion.
TS13: head	Strong	Regionally restricted	6C	Expression is rostral to the region of neural tube fusion in the cranial region.
TS13: embryo	Present	Regionally restricted	6C	Expression is in the caudal region.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch	Strong		6D
TS14: notochord	Strong		6D
TS14: somite	Strong	Regionally restricted	6D	Expression is at the inter-somitic junctions.
TS14: embryo	Present		6D	Expression is throughout.
TS14: branchial arch	Present		6D

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Strong	Regionally restricted	6E	Expression is at the superior aspect.
TS15: 1st branchial arch	Strong	Regionally restricted	6E	Expression is at the lateral aspects.
TS15: 2nd branchial arch	Strong	Regionally restricted	6E	Expression is at the lateral aspects.

Structure	Level	Pattern	Image	Note
TS16: limb	Weak	Regionally restricted	6G	Expression is at the leading edges.
TS16: branchial arch	Weak		6G
TS16: tail somite	Weak	Regionally restricted	6G	Expression is in the most caudal portion.
TS16: embryo	Trace		6G