Slc12a5 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:288517 Spoljaric I, et al., KCC2-Mediated Cl(-) Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice. Cell Rep. 2019 Jan 29;26(5):1073-1081.e3
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6441796
Gene symbol:	Slc12a5
Gene name:	solute carrier family 12, member 5

Antibody:	anti-KCC2 (#07-432)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Results

1B +/+ ©

Specimen 1B +/+: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: hippocampus CA3	Present	Single cells	1B +/+

1B -/- ©

Specimen 1B -/-: embryonic day 18.5; Slc12a5^tm1Hsav/Slc12a5^tm1Hsav (more

)

Structure	Level	Pattern	Image	Note
TS26: hippocampus CA3	Absent		1B -/-

2A ©

2A KCC2 ©

2A MERGE ©

Specimen 2A: embryonic day 18.5; Gad1^tm1.1Tama/Gad1⁺ (more

)
Note: Double labeled: red - Slc12a5; green - Gad1 reporter (Gad67; assay MGI:6441798)

Structure	Level	Pattern	Image	Note
TS26: hippocampus CA3	Present		2A , 2A KCC2 , 2A MERGE	Expression is seen in interneurons and pyramidal neurons.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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