Gene Expression Data
Assay Details
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Assay
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Reference:
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J:278913
Meyer G, et al., Cajal-Retzius neurons are required for the development of the human hippocampal fissure. J Anat. 2019 Sep;235(3):569-589
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6441740
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Gene symbol:
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Reln
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Gene name:
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reelin
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Antibody:
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anti-Reelin 142
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
8A:
embryonic day 15.0
(more  )
Specimen
8A:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical hem |
Present |
Regionally restricted |
8A
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Cajal-Retzius neurons leave the hem and assemble in the hippocampal fissure, which begins to invaginate.
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Specimen
8B:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Trp73tm1Fmc/Trp73tm1Fmc
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical hem |
Strong |
Regionally restricted |
8B
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High expression in the hem, but Cajal-Retzius neurons do not leave the hem, and hippocampal fissure fails to invaginate.
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Specimen
8C:
postnatal day 9
(more )
Specimen
8C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 9
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampal fissure |
Strong |
Regionally restricted |
8C
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Hippocampal fissure is densely populated with Cajal-Retzius neurons.
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Specimen
8D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Trp73tm1Fmc/Trp73tm1Fmc
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Age:
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postnatal day 9
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampal fissure |
Absent |
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8D
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Authors report absence of Cajal-Retzius neurons in mutant hippocampal fissure.
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| TS28: dentate gyrus molecular layer |
Present |
Regionally restricted |
8D
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Expression in interneurons in strata oriens and lacunosum-moleculare of CA1 and dentate molecular layer
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| TS28: hippocampus CA1 |
Present |
Regionally restricted |
8D
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Expression in interneurons in strata oriens and lacunosum-moleculare of CA1 and dentate molecular layer
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Specimen
8G:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Trp73tm1Fmc/Trp73tm1Fmc
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus stratum lacunosum |
Present |
Regionally restricted |
8G
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Expression in interneurons irregularly clustered in stratum lacunosum-moleculare, following CA1 folds.
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Specimen
9E:
postnatal day 1
(more )
Specimen
9E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: hippocampal fissure |
Present |
Regionally restricted |
9E
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Expression in interneurons in dorsal hippocampal fissure.
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Specimen
9F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Trp73tm1Fmc/Trp73tm1Fmc
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: hippocampal fissure |
Weak |
Clusters |
9F
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Expression in only a small cluster of Cajal-Retzius neurons where hippocampal fissure rudiment is invaginated.
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| TS27: hippocampus |
Weak |
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9F
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Overall amount of expression in hippocampus was significantly lower than in wild type.
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