Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36064
Mastick GS, et al., Early deletion of neuromeres in Wnt-1-/- mutant mice: evaluation by morphological and molecular markers. J Comp Neurol. 1996 Oct 14;374(2):246-58
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6441536
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Gene symbol:
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Tubb3
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Gene name:
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tubulin, beta 3 class III
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Antibody:
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anti-TuJ1
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Detection system:
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Secondary antibody
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Results
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Specimen
2A:
embryonic day 9.5
(more  )
Note: Embryo with 25-30 somites.
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo with 25-30 somites.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt1tm1Brd/Wnt1tm1Brd
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo with 25-30 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: central nervous system |
Present |
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2B
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Expression is in neurons and their axons. Expression is altered in this mutant due to neuronal defects.
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| TS15: peripheral nervous system |
Present |
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2B
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Expression is in neurons and their axons. Expression is altered in this mutant due to neuronal defects.
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Specimen
2C:
embryonic day 9.5
(more )
Note: Embryo with 25-30 somites.
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo with 25-30 somites.
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Specimen
2D:
embryonic day 9.5
(more )
Note: Embryo with 25-30 somites.
Specimen
2D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo with 25-30 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future brain |
Present |
Regionally restricted |
2D
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Expression is altered in this mutant due to neuronal defects (lacking mesencephalon and rhombomere 1).
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| TS15: rhombomere 2 |
Present |
Regionally restricted |
2D
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Expression in this mutant is similar to wild-type.
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| TS15: rhombomere 3 |
Present |
Regionally restricted |
2D
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Expression in this mutant is similar to wild-type.
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| TS15: rhombomere 4 |
Present |
Regionally restricted |
2D
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Expression in this mutant is similar to wild-type.
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| TS15: rhombomere 5 |
Present |
Regionally restricted |
2D
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Expression in this mutant is similar to wild-type.
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| TS15: rhombomere 6 |
Present |
Regionally restricted |
2D
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Expression in this mutant is similar to wild-type.
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Specimen
6A:
embryonic day 9.5
(more )
Note: Embryo with 25-30 somites.
Specimen
6A:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Embryo with 25-30 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
6A
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Expression is in neurons and their axons. Shown are the medial longitudinal fasciculus bundles and the tract of the mesencephalic nucleus of the trigeminal nerve.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt1tm1Brd/Wnt1tm1Brd
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Embryo with 25-30 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
6B
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Expression in the axon bundles of this mutant is the same as in wild type.
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Analysis Tools