Antibody:	anti-IGSF4
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3a	Diffuse expression is seen in the dorsal telencephalic wall.

Structure	Level	Pattern	Image	Note
TS22: cortical subplate	Strong		3b

Structure	Level	Pattern	Image	Note
TS22: cortical subplate	Strong		3c

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Present		3d
TS24: cortical plate	Present		3d
TS24: cortical subplate	Present		3d
TS24: cerebral cortex intermediate zone	Present	Regionally restricted	3d	Expression is seen in the outer half of the intermediate zone; it is not seen in the innermost half.
TS24: cerebral cortex ventricular layer	Present	Regionally restricted	3d	Expression is seen in the outermost part of the ventricular zone.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Present		3e

Structure	Level	Pattern	Image	Note
TS24: cortical subplate	Present		3f

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Present	Regionally restricted	3g	Expression is seen in the outer half of the intermediate zone; it is not seen in the innermost half.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present		4a
TS26: cerebral cortex marginal zone	Present		4a
TS26: cerebral cortex ventricular layer	Present		4a	Expression in this structure at this age is less intense than that seen earlier.
TS26: cortical plate	Present		4a	Expression in this structure at this age is less intense than that seen earlier.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present		4b

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present	Single cells	4c	Expression is seen on radially oriented fibers.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Weak	Diffuse	4d

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak		4e	Specific, zonal patterns are no longer observed.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak	Widespread
TS28: lung	Weak

Structure	Level	Pattern	Image	Note
TS27: cerebellum molecular layer	Present	Regionally restricted	5a	Expression is seen in the migrating granule cells located in the molecular layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum molecular layer	Present	Regionally restricted	5b	Expression is seen in the migrating granule cells located in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present	Regionally restricted	5c	Expression is seen in the migrating granule cells located in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present	Regionally restricted	5d	Expression is seen in the migrating granule cells located in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Strong	Diffuse	5e
TS28: cerebellum molecular layer	Strong	Diffuse	5e
TS28: cerebellum Purkinje cell layer	Absent		5e	No expression is seen in the cytoplasm or dendrites of Purkinje cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Strong	Diffuse	5f

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Single cells	5g	There is transient expression in Golgi type II cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Single cells	5h	There is transient expression in Golgi type II cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		5i	Expression levels are decreased from those seen earlier.

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		6a
TS26: peripheral nerve	Present		6a	Expression is seen in peripheral nerve fibers.

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		6b

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present
TS20: peripheral nerve	Present			Expression is seen in peripheral nerve fibers.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Strong		6c	There is intense localization at cell-cell contact regions.

Structure	Level	Pattern	Image	Note
TS24: cortical subplate	Present		7a	Expression is seen on the cell surface of neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present	Regionally restricted	7c	Expression is seen in the migrating granule cells located in the molecular layer.

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		7e	Expression is seen on the cell surface of neurons.