Probe:	Hey2 probe7
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Present		1B left

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Present		1B right

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present		2A whole mount

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present		2A section

Structure	Level	Pattern	Image	Note
TS13: primitive ventricle cardiac muscle	Present	Regionally restricted	2B whole mount	Expression was detected in the compact layer and contiguous trabeculae of ventricular free wall.

Structure	Level	Pattern	Image	Note
TS13: primitive ventricle cardiac muscle	Present	Regionally restricted	2B section	Expression was detected in the compact layer and contiguous trabeculae of ventricular free wall.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Present	Regionally restricted	2C whole mount	Expression was detected in the compact layer and contiguous trabeculae of ventricular free wall.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Present	Regionally restricted	2C section	Expression was detected in the compact layer and contiguous trabeculae of ventricular free wall.

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Present	Regionally restricted	2D whole mount	Expression was detected in the compact layer and contiguous trabeculae of ventricular free wall.
TS19: interventricular septum	Present		2D whole mount

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Present	Regionally restricted	2D section	Expression was detected in the compact layer and contiguous trabeculae of ventricular free wall.
TS19: interventricular septum	Present		2D section

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		4E left wt/wt
TS15: primitive ventricle cardiac muscle	Present		4E left wt/wt
TS15: somite	Present		4E left wt/wt

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal endocardial lining	Present		4E center wt/wt
TS15: primitive ventricle cardiac muscle	Present		4E center wt/wt

Structure	Level	Pattern	Image	Note
TS15: endocardial lining	Present		4E right wt/wt
TS15: primitive ventricle cardiac muscle	Present		4E right wt/wt

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Weak		4E left Hey2 -211kdelta/delta	Expression was downregulated in the mutant ventricular myocardium.
TS15: branchial arch	Present		4E left Hey2 -211kdelta/delta	Expression in other tissues was retained in the mutant.
TS15: somite	Present		4E left Hey2 -211kdelta/delta	Expression in other tissues was retained in the mutant.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Weak		4E center Hey2 -211kdelta/delta	Expression was downregulated in the mutant ventricular myocardium.
TS15: atrioventricular canal endocardial lining	Present		4E center Hey2 -211kdelta/delta	Expression in the endocardium was retained in the mutant.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Weak		4E right Hey2 -211kdelta/delta	Expression was downregulated in the mutant ventricular myocardium.
TS15: endocardial lining	Present		4E right Hey2 -211kdelta/delta	Expression in the endocardium was retained in the mutant.

Structure	Level	Pattern	Image	Note
TS15: endocardial lining	Present		6E wt/wt left
TS15: primitive ventricle cardiac muscle	Present		6E wt/wt left

Structure	Level	Pattern	Image	Note
TS15: endocardial lining	Present		6E wt/wt right
TS15: primitive ventricle cardiac muscle	Present		6E wt/wt right

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Weak		6E ko/ko left	Expression was downregulated in the mutant ventricular myocardium.
TS15: endocardial lining	Present		6E ko/ko left	Expression in the endocardium was retained in the mutant.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Weak		6E ko/ko right	Expression was downregulated in the mutant ventricular myocardium.
TS15: endocardial lining	Present		6E ko/ko right	Expression in the endocardium was retained in the mutant.