Gene Expression Data
Assay Details
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Assay
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Reference:
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J:199462
Probst S, et al., The hedgehog target Vlk genetically interacts with Gli3 to regulate chondrocyte differentiation during mouse long bone development. Differentiation. 2013 Apr-Jun;85(4-5):121-30
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6438080
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Gene symbol:
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Pkdcc
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Gene name:
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protein kinase domain containing, cytoplasmic
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Probe:
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Vlk probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A E9.5:
embryonic day 9.5
(more  )
Specimen
1A E9.5:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1A E10.5:
embryonic day 10.5
(more )
Specimen
1A E10.5:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
1A E10.5
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Expression restricted to proximal mesenchyme.
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Specimen
1A E11.0:
embryonic day 11.0
(more )
Specimen
1A E11.0:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
1A E11.0
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Expression initially higher in anterior mesenchyme, and excluded from developing handplate and core mesenchyme.
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Specimen
1A E11.5:
embryonic day 11.5
(more )
Specimen
1A E11.5:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1A E12.5:
embryonic day 12.5
(more )
Specimen
1A E12.5:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb mesenchyme |
Present |
Regionally restricted |
1A E12.5
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Expression in anterior and posterior domains, and excluded from core mesenchyme.
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| TS20: hand |
Absent |
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1A E12.5
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Specimen
1B dorsal:
embryonic day 13.5
(more )
Specimen
1B dorsal:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forearm |
Strong |
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1B dorsal
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High expression in presumptive stylopod and zeugopod.
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| TS21: upper arm |
Strong |
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1B dorsal
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High expression in presumptive stylopod and zeugopod.
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| TS21: forelimb digit |
Absent |
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1B dorsal
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No significant expression in developing autopod and digit primordia.
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| TS21: hand |
Absent |
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1B dorsal
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No significant expression in developing autopod and digit primordia.
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Specimen
1B ventral:
embryonic day 13.5
(more )
Specimen
1B ventral:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forearm |
Strong |
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1B ventral
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High expression in presumptive stylopod and zeugopod.
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| TS21: upper arm |
Strong |
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1B ventral
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High expression in presumptive stylopod and zeugopod.
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| TS21: forelimb digit |
Absent |
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1B ventral
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No significant expression in developing autopod and digit primordia.
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| TS21: hand |
Absent |
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1B ventral
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No significant expression in developing autopod and digit primordia.
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Specimen
1C humerus:
embryonic day 16.5
(more )
Specimen
1C humerus:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
1C humerus
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Expression in columnar chondrocytes.
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Specimen
1C ulna/radius D:
embryonic day 16.5
(more )
Specimen
1C ulna/radius D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: radius |
Present |
Regionally restricted |
1C ulna/radius D
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Expression in columnar chondrocytes.
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| TS24: ulna |
Present |
Regionally restricted |
1C ulna/radius D
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Expression in columnar chondrocytes.
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Specimen
1C ulna/radius P:
embryonic day 16.5
(more )
Specimen
1C ulna/radius P:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: radius |
Present |
Regionally restricted |
1C ulna/radius P
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Expression in ossification centers.
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| TS24: ulna |
Present |
Regionally restricted |
1C ulna/radius P
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Expression in ossification centers.
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