About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:262369 Taya Y, et al., Migration of lymphatic endothelial cells and lymphatic vascular development in the craniofacial region of embryonic mice. Int J Dev Biol. 2018;62(4-5):293-301
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6437762
Gene symbol: Flt4
Gene name: FMS-like tyrosine kinase 4
Antibody: anti-VEGFR3 (AF743)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1D: embryonic day 9.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS15: cardinal vein Present Regionally restricted 1D Expression is in endothelial cells.
TS15: trunk Present Single cells 1D Expression is in cells that budded from the cardinal vein or are scattered from the cardinal vein.

Specimen 1J: embryonic day 10.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS17: trunk Present Single cells 1J Expression is in cells near the cardinal vein.

Specimen 1P: embryonic day 11.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 1P Expression is in lymph sacs near the cardinal vein.

Specimen 2A: embryonic day 11.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS19: trunk Present Regionally restricted 2Aa , 2Ab , 2A Expression is in lymphatic endothelial cells from the cardinal veins to the craniofacial region.
TS19: lymph sac Present 2Ab , 2A

Specimen 3A: embryonic day 10.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 34 sagittal serial sections.
Structure Level Pattern Image Note
TS17: trunk Present Single cells 3C , 3A , 3B Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch Present Single cells 3C , 3B , 3A Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 3D: embryonic day 10.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 34 sagittal serial sections.
Structure Level Pattern Image Note
TS17: trunk Present Single cells 3D Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch Present Single cells 3D Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 3E: embryonic day 10.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 34 sagittal serial sections.
Structure Level Pattern Image Note
TS17: trunk Present Single cells 3E Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch Present Single cells 3E Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 3F: embryonic day 10.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 34 sagittal serial sections.
Structure Level Pattern Image Note
TS17: trunk Present Single cells 3F Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch Present Single cells 3F Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 3G: embryonic day 10.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 34 sagittal serial sections.
Structure Level Pattern Image Note
TS17: trunk Present Single cells 3G Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch Present Single cells 3G Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 4A: embryonic day 11.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 45 sagittal serial sections.
Structure Level Pattern Image Note
TS19: trunk Present Single cells 4A , 4C , 4B Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch Present Single cells 4B , 4C , 4A Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 4D: embryonic day 11.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 45 sagittal serial sections.
Structure Level Pattern Image Note
TS19: trunk Present Single cells 4D Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch Present Single cells 4D Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 4E: embryonic day 11.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 45 sagittal serial sections.
Structure Level Pattern Image Note
TS19: trunk Present Single cells 4E Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch Present Single cells 4E Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 4F: embryonic day 11.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 45 sagittal serial sections.
Structure Level Pattern Image Note
TS19: trunk Present Single cells 4F Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch Present Single cells 4F Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 4G: embryonic day 11.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759). Three-dimensional image constructed from 45 sagittal serial sections.
Structure Level Pattern Image Note
TS19: trunk Present Single cells 4G Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch Present Single cells 4G Expression is in lymphatic endothelial cells entering the mandibular arch.

Specimen 5A: embryonic day 9.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS15: head Present Single cells 5B , 5A Expression is in lymphatic endothelial cells in the craniofacial region.

Specimen 5C: embryonic day 10.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS17: 1st branchial arch Present Single cells 5D , 5C Expression is in lymphatic endothelial cells.

Specimen 5E: embryonic day 11.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS19: 1st branchial arch Present Regionally restricted 5F , 5E Expression is in lymphatic endothelial cell masses.

Specimen 5G: embryonic day 12.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS20: lymph sac Present Regionally restricted 5G , 5H Expression is in unlumenized lymph sacs in the craniofacial region.

Specimen 5I: embryonic day 14.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS22: lymph sac Present Regionally restricted 5I , 5J Expression is in lumenized lymph sacs in the craniofacial region.

Specimen 7A: embryonic day 12.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS20: lymph sac Present Regionally restricted 7A Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS20: mandibular process Present Regionally restricted 7A Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.

Specimen 7E: embryonic day 14.5 (more )
Note: Double labeled: green - Flt4; red - Prox1 (assay MGI:6437759).
Structure Level Pattern Image Note
TS22: lower jaw Present Regionally restricted 7E Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS22: lymph sac Present Regionally restricted 7E Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
04/16/2024
MGI 6.23
The Jackson Laboratory