Antibody:	anti-Prox1 (#11-002)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS15: cardinal vein	Present	Single cells	1A , 1B	Expression is in a subpopulation of venous endothelial cells in the lateral wall.
TS15: trunk	Present	Single cells	1B , 1A	Expression is in cells that budded from the cardinal vein or are scattered from the cardinal vein.

Structure	Level	Pattern	Image	Note
TS15: cardinal vein	Present	Single cells	1C	Expression is in a subpopulation of venous endothelial cells in the lateral wall.
TS15: trunk	Present	Single cells	1C	Expression is in cells that budded from the cardinal vein or are scattered from the cardinal vein.

Structure	Level	Pattern	Image	Note
TS15: cardinal vein	Present	Single cells	1D	Expression is in a subpopulation of venous endothelial cells in the lateral wall.
TS15: trunk	Present	Single cells	1D	Expression is in cells that budded from the cardinal vein or are scattered from the cardinal vein.

Structure	Level	Pattern	Image	Note
TS15: cardinal vein	Present	Single cells	1E	Expression is in a subpopulation of venous endothelial cells in the lateral wall.
TS15: trunk	Present	Single cells	1E	Expression is in cells that budded from the cardinal vein or are scattered from the cardinal vein.

Structure	Level	Pattern	Image	Note
TS15: cardinal vein	Present	Single cells	1F	Expression is in a subpopulation of venous endothelial cells in the lateral wall.
TS15: trunk	Present	Single cells	1F	Expression is in cells that budded from the cardinal vein or are scattered from the cardinal vein.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	1G , 1H	Expression is in cells near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	1I	Expression is in cells near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	1J	Expression is in cells near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	1K	Expression is in cells near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	1L	Expression is in cells near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present	Regionally restricted	1N , 1M	Expression is in lymph sacs near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present	Regionally restricted	1O	Expression is in lymph sacs near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present	Regionally restricted	1P	Expression is in lymph sacs near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present	Regionally restricted	1Q	Expression is in lymph sacs near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present	Regionally restricted	1R	Expression is in lymph sacs near the cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: trunk	Present	Regionally restricted	2Ab , 2Aa , 2A	Expression is in lymphatic endothelial cells from the cardinal veins to the craniofacial region.
TS19: lymph sac	Present		2Ab , 2A

Structure	Level	Pattern	Image	Note
TS19: trunk	Present	Regionally restricted	2Bd , 2Bc , 2B	Expression is in lymphatic endothelial cells from the cardinal veins to the craniofacial region.
TS19: lymph sac	Present		2B , 2Bd

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	3A , 3B , 3C	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch	Present	Single cells	3B , 3C , 3A	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	3D	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch	Present	Single cells	3D	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	3E	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch	Present	Single cells	3E	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	3F	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch	Present	Single cells	3F	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Single cells	3G	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS17: 1st branchial arch	Present	Single cells	3G	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS19: trunk	Present	Single cells	4A , 4B , 4C	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch	Present	Single cells	4A , 4C , 4B	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS19: trunk	Present	Single cells	4D	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch	Present	Single cells	4D	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS19: trunk	Present	Single cells	4E	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch	Present	Single cells	4E	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS19: trunk	Present	Single cells	4F	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch	Present	Single cells	4F	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS19: trunk	Present	Single cells	4G	Expression is in lymphatic endothelial cell streams from the middle of the trunk to the adjacent mandibular arches.
TS19: 1st branchial arch	Present	Single cells	4G	Expression is in lymphatic endothelial cells entering the mandibular arch.

Structure	Level	Pattern	Image	Note
TS15: head	Present	Single cells	5A , 5B	Expression is in lymphatic endothelial cells in the craniofacial region.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Single cells	5C , 5D	Expression is in lymphatic endothelial cells.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	5F , 5E	Expression is in lymphatic endothelial cell masses.

Structure	Level	Pattern	Image	Note
TS20: lymph sac	Present	Regionally restricted	5H , 5G	Expression is in unlumenized lymph sacs in the craniofacial region.

Structure	Level	Pattern	Image	Note
TS22: lymph sac	Present	Regionally restricted	5I , 5J	Expression is in lumenized lymph sacs in the craniofacial region.

Structure	Level	Pattern	Image	Note
TS20: lymph sac	Present	Regionally restricted	7A	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS20: mandibular process	Present	Regionally restricted	7A	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.

Structure	Level	Pattern	Image	Note
TS20: lymph sac	Present	Regionally restricted	7B	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS20: mandibular process	Present	Regionally restricted	7B	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.

Structure	Level	Pattern	Image	Note
TS20: lymph sac	Present	Regionally restricted	7C	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS20: mandibular process	Present	Regionally restricted	7C	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.

Structure	Level	Pattern	Image	Note
TS20: lymph sac	Present	Regionally restricted	7D	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS20: mandibular process	Present	Regionally restricted	7D	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.

Structure	Level	Pattern	Image	Note
TS22: lower jaw	Present	Regionally restricted	7E	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS22: lymph sac	Present	Regionally restricted	7E	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.

Structure	Level	Pattern	Image	Note
TS22: lower jaw	Present	Regionally restricted	7F	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS22: lymph sac	Present	Regionally restricted	7F	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.

Structure	Level	Pattern	Image	Note
TS22: lower jaw	Present	Regionally restricted	7G	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS22: lymph sac	Present	Regionally restricted	7G	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.

Structure	Level	Pattern	Image	Note
TS22: lower jaw	Present	Regionally restricted	7H	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.
TS22: lymph sac	Present	Regionally restricted	7H	Expression is in lymphatic endothelial cells, including individual, aggregated, and forming lymph sacs and lymphatic vessels in the sublingual regions of the mandibular arches.