Gene Expression Data
Assay Details
Assay
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Reference:
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J:185947
Parisi I, et al., Regulation of Merkel cell development by Pax6. Int J Dev Biol. 2012;56(5):341-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6437494
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Antibody:
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anti-Pax6
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2D/F:
embryonic day 18.5
(more )
Note: Double labeled: green - Pax6; red - Krt8 (assay MGI:6437499).
Specimen
2D/F:
(close )
Genetic Background:
|
involves: 102 * C3H * CBA/Ca
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
|
Double labeled: green - Pax6; red - Krt8 (assay MGI:6437499).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: vibrissa follicle |
Present |
Regionally restricted |
2F
,
2D
|
Expression overlapping with Krt8 expression in Merkel cells, predominantly localized in cytoplasm.
|
Specimen
2G/I:
embryonic day 18.5
(more )
Note: Double labeled: green - Pax6; red - Krt20 (assay MGI:6437503).
Specimen
2G/I:
(close )
Genetic Background:
|
involves: 102 * C3H * CBA/Ca
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Pax6; red - Krt20 (assay MGI:6437503).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: vibrissa follicle |
Present |
Regionally restricted |
2I
,
2G
|
Expression overlapping with Krt20 expression in Merkel cells.
|
Specimen
2J/L:
postnatal day 4
(more )
Note: Double labeled: green - Pax6; red - Krt20 (assay MGI:6437503).
Specimen
2J/L:
(close )
Genetic Background:
|
involves: 102 * C3H * CBA/Ca
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Pax6; red - Krt20 (assay MGI:6437503).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair outer root sheath |
Present |
Regionally restricted |
2J
,
2L
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Expression in Merkel cells in outer root sheath of hair follicle, co-localizing with Krt20 expression.
|
Specimen
2J'/L':
postnatal day 4
(more )
Note: Double labeled: green - Pax6; red - Krt20 (assay MGI:6437503).
Specimen
2J'/L':
(close )
Genetic Background:
|
involves: 102 * C3H * CBA/Ca
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Pax6; red - Krt20 (assay MGI:6437503).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair outer root sheath |
Present |
Regionally restricted |
2J'
,
2L'
|
Expression partially relocated into nuclei of Merkel cells.
|
Specimen
S1A/C:
postnatal day 4
(more )
Note: Double labeled: green - Pax6; red - Vip (assay MGI:6437506).
Specimen
S1A/C:
(close )
Genetic Background:
|
involves: 102 * C3H * CBA/Ca
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Pax6; red - Vip (assay MGI:6437506).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair outer root sheath |
Present |
Regionally restricted |
S1C
,
S1A
|
Expression does not overlap with that of Vip.
|
|