Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143023
Coumailleau P, et al., Sim1 and Sim2 expression during chick and mouse limb development. Int J Dev Biol. 2009;53(1):149-57
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6437448
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Gene symbol:
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Sim2
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Gene name:
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single-minded family bHLH transcription factor 2
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Probe:
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Sim2 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
E10 forelimb not shown:
embryonic day 10.0
(more  )
Specimen
E10 forelimb not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud mesenchyme |
Present |
Regionally restricted |
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First expression in limb muscle observed in E10 forelimb and E11 hind limbs.
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Specimen
E11 hindlimb not shown:
embryonic day 11.0
(more )
Specimen
E11 hindlimb not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindlimb bud mesenchyme |
Present |
Regionally restricted |
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First expression in limb muscle observed in E10 forelimb and E11 hind limbs.
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Specimen
5E:
embryonic day 10.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Absent |
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5E
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No expression in limb level somites or early migrating limb cells.
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Specimen
5G:
embryonic day 10.5
(more )
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
5G
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Faint expression in ventral and dorsal muscle masses. Expression clearly enhanced in ventral muscle progenitors compared to Pax3 expression.
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Specimen
5H:
embryonic day 11.0
(more )
Specimen
5H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
5H
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Faint expression in ventral and dorsal muscle masses. Expression clearly enhanced in ventral muscle progenitors compared to Pax3 expression. Additional expression in domain which is non-myogenic region.
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Specimen
6A:
embryonic day 11.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
6A
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Expression in myogenic cells of distal forelimb.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pax3tm1Buck/Pax3tm1Buck
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Specimen
6E:
embryonic day 11.5
(more )
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
6E
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Mainly ventral expression, complementary to that of Myod1.
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pax3tm1Buck/Pax3tm1Buck
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Specimen
6I:
embryonic day 11.5
(more )
Specimen
6I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
6I
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Non-myogenic expression in posterior of proximal region.
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Specimen
6J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pax3tm1Buck/Pax3tm1Buck
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
6J
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Non-myogenic expression in posterior of proximal region.
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Specimen
E12.5 not shown:
embryonic day 12.5
(more )
Specimen
E12.5 not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb mesenchyme |
Present |
Regionally restricted |
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Expression enhanced in ventral muscle masses of forelimbs and hindlimbs.
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| TS20: hindlimb mesenchyme |
Present |
Regionally restricted |
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Expression enhanced in ventral muscle masses of forelimbs and hindlimbs.
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Specimen
E14.5 not shown:
embryonic day 14.5
(more )
Specimen
E14.5 not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Analysis Tools