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Gene Expression Data
Assay Details
Assay
Reference: J:48168 van Kleffens M, et al., mRNA expression patterns of the IGF system during mouse limb bud development, determined by whole mount in situ hybridization. Mol Cell Endocrinol. 1998 Mar 16;138(1-2):151-61
Assay type: RNA in situ
MGI Accession ID: MGI:6437398
Gene symbol: Igfbp5
Gene name: insulin-like growth factor binding protein 5
Probe: Igfbp5 cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5J: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present 5J

Specimen 5K: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hindlimb interdigital region Present Regionally restricted 5K Sequential expression was detected in the proximal interdigital region.
TS21: hindlimb phalanx pre-cartilage condensation Present Regionally restricted 5K Sequential expression was detected in the phalangeal region; halfway the phalangeal region, more proximally, to disappear finally, except the most proximal signal.

Specimen 5L: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: hindlimb interdigital region Present Regionally restricted 5L Expression was detected in the proximal part of the interdigital zone.
TS22: hindlimb interphalangeal joint Present 5L

Specimen not shown: embryonic day 9.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS15: forelimb bud ectoderm Present
TS15: forelimb bud presumptive apical ectodermal ridge Present

Specimen not shown: embryonic day 10.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present
TS17: forelimb bud ectoderm Present

Specimen not shown: embryonic day 11.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS19: forelimb bud ectoderm Present
TS19: handplate apical ectodermal ridge Present
TS19: forelimb bud Weak Regionally restricted A weak signal was observed in the border region between the paddle and arm, presumably in muscle primordia.

Specimen not shown: embryonic day 12.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS20: forelimb Present Regionally restricted Signal at E12.5 was similar to E11.5.

Specimen not shown: embryonic day 13.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS21: forelimb interdigital region Present
TS21: forelimb digit pre-cartilage condensation Present Regionally restricted Expression was detected in proximal regions.

Specimen not shown: embryonic day 14.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS22: hand Present Graded Expression was detected in a thin region bordering the interdigital zone. The signal was graded, with strongest signal proximally.
TS22: forelimb cartilage condensation Present Regionally restricted The borders of the bone primordia were stained less intensely.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory