Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:48168
van Kleffens M, et al., mRNA expression patterns of the IGF system during mouse limb bud development, determined by whole mount in situ hybridization. Mol Cell Endocrinol. 1998 Mar 16;138(1-2):151-61
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6437395
|
|
Gene symbol:
|
Igfbp4
|
|
Gene name:
|
insulin-like growth factor binding protein 4
|
|
Probe:
|
Igfbp4 cDNA4
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
5G:
embryonic day 10.5
(more  )
Specimen
5G:
(close  )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
5G
|
Expression was detected in posterior proximal mesoderm, but in a smaller region compared to E9.5.
|
Specimen
5H:
embryonic day 12.5
(more )
Specimen
5H:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5I:
embryonic day 14.5
(more )
Specimen
5I:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindlimb interdigital region |
Present |
Regionally restricted |
5I
|
Expression was detected in the proximal interdigital area.
|
| TS22: hindlimb |
Present |
Regionally restricted |
5I
|
Expression was detected in proximal posterior hindlimb.
|
| TS22: hindlimb interphalangeal joint |
Present |
Regionally restricted |
5I
|
A sequential expression pattern was noted in the pre-phalangeal joint areas. Signal was found between the two developing proximal phalanges, in the future joints.
|
Specimen
not shown:
embryonic day 9.5
(more )
Note: As per schematic overview of expression pattern in Fig. 1.
Specimen
not shown:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
As per schematic overview of expression pattern in Fig. 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud mesenchyme |
Present |
Regionally restricted |
|
Expression was detected in the posterior proximal mesoderm, or ZPA region.
|
Specimen
not shown:
embryonic day 11.5
(more )
Note: As per schematic overview of expression pattern in Fig. 1.
Specimen
not shown:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
As per schematic overview of expression pattern in Fig. 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
|
Both posterior and anterior mesodermal cell layers were stained, the posterior signal being most intense.
|
Specimen
not shown:
embryonic day 13.5
(more )
Note: As per schematic overview of expression pattern in Fig. 1.
Specimen
not shown:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
As per schematic overview of expression pattern in Fig. 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb interdigital region |
Strong |
Non-Uniform |
|
At E13.5 the overall interdigital signal decreased, but a strong expression was located in a small most proximal interdigital region.
|
Specimen
not shown:
embryonic day 14.5
(more )
Note: As per schematic overview of expression pattern in Fig. 1.
Specimen
not shown:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
As per schematic overview of expression pattern in Fig. 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb interdigital region |
Present |
Regionally restricted |
|
The interdigital signal became located towards the borders of the interdigital zone, in mesenchymal cells surrounding the proximal phalangeal bone primordia.
|
| TS22: forelimb |
Present |
Regionally restricted |
|
Expression was detected in a small posterior proximal region.
|
|
Analysis Tools