Gene Expression Data
Assay Details
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Assay
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Reference:
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J:48168
van Kleffens M, et al., mRNA expression patterns of the IGF system during mouse limb bud development, determined by whole mount in situ hybridization. Mol Cell Endocrinol. 1998 Mar 16;138(1-2):151-61
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6437383
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Gene symbol:
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Igfbp3
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Gene name:
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insulin-like growth factor binding protein 3
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Probe:
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Igfbp3 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5D:
embryonic day 11.5
(more  )
Specimen
5D:
(close  )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: blood vessel |
Present |
Regionally restricted |
5D
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Expression was detected in blood vessels in the forelimb in the middle of the paddle.
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| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
5D
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Expression was detected in the posterior and anterior proximal mesoderm.
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Specimen
5E:
embryonic day 12.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb interdigital region |
Present |
Regionally restricted |
5E
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Expression was detected in the proximal interdigital region and the borders of the interdigital zones.
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Specimen
5F:
embryonic day 14.5
(more )
Specimen
5F:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown:
embryonic day 9.5
(more )
Note: As per schematic overview of expression pattern in Fig. 1.
Specimen
not shown:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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As per schematic overview of expression pattern in Fig. 1.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: blood vessel |
Present |
Regionally restricted |
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Expression was found in the middle part of the forelimb, presumably in developing blood vessels.
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| TS15: forelimb bud |
Present |
Regionally restricted |
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Expression was found in the middle part of the forelimb, presumably in developing blood vessels.
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Specimen
not shown:
embryonic day 10.5
(more )
Note: As per schematic overview of expression pattern in Fig. 1.
Specimen
not shown:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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As per schematic overview of expression pattern in Fig. 1.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: blood vessel |
Present |
Regionally restricted |
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Expression was found in the middle part of the forelimb, presumably in developing blood vessels.
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| TS17: forelimb bud |
Present |
Regionally restricted |
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Expression was found in the middle part of the forelimb, presumably in developing blood vessels.
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| TS17: marginal vein of forelimb |
Present |
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| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
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Expression was found in the posterior and anterior proximal mesoderm, strongest anteriorly.
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Specimen
not shown:
embryonic day 13.5
(more )
Note: As per schematic overview of expression pattern in Fig. 1.
Specimen
not shown:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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As per schematic overview of expression pattern in Fig. 1.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb interdigital region mesenchyme |
Present |
Regionally restricted |
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Expression was found in a similar pattern to E12.5. Staining was also found in distal mesodermal cells, bordering the region where separation of the digits has already taken place.
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