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Gene Expression Data
Assay Details
Assay
Reference: J:48168 van Kleffens M, et al., mRNA expression patterns of the IGF system during mouse limb bud development, determined by whole mount in situ hybridization. Mol Cell Endocrinol. 1998 Mar 16;138(1-2):151-61
Assay type: RNA in situ
MGI Accession ID: MGI:6437377
Gene symbol: Igfbp2
Gene name: insulin-like growth factor binding protein 2
Probe: Igfbp2 cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: forelimb bud presumptive apical ectodermal ridge Weak 5A
TS15: forelimb bud mesenchyme Present Regionally restricted 5A Expression was detected in the proximal mesoderm. Expression was most intense anteriorly.

Specimen 5B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hindlimb interdigital region Present 5B Expression becomes stronger towards the distal ectoderm and shows a sequential expression pattern starting between digit III and IV.

Specimen 5C: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: hindlimb interdigital region Present Regionally restricted 5C Expression was detected in the borders of the interdigital region.
TS22: hindlimb digit mesenchyme Present Regionally restricted 5C Expression was detected in the mesodermal cells surrounding the distal part of the pre-phalanges.
TS22: hindlimb mesenchyme Present Regionally restricted 5C Expression was most intense distally, in the region where the basal membrane is located.

Specimen not shown: embryonic day 10.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS17: forelimb bud Present

Specimen not shown: embryonic day 11.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present
TS19: marginal vein of forelimb Present
TS19: forelimb bud Present Regionally restricted The signal was still localized anteriorly.

Specimen not shown: embryonic day 12.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS20: forelimb Present Regionally restricted Ectodermal signal remains intense until E12.5.
TS20: marginal vein of forelimb Weak
TS20: forelimb interdigital region mesenchyme Present

Specimen not shown: embryonic day 13.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS21: forelimb interdigital region mesenchyme Present Regionally restricted Expression becomes stronger towards the distal ectoderm and shows a sequential expression pattern starting between digit III and IV.
TS21: interdigital region between forelimb digits 3 and 4 Present

Specimen not shown: embryonic day 14.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS22: forelimb interdigital region Present Regionally restricted Expression was found in the proximal interdigital regions.
TS22: forelimb mesenchyme Present Regionally restricted Expression was located in the mesoderm surrounding the skeletal primordia.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory