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Gene Expression Data
Assay Details
Assay
Reference: J:48168 van Kleffens M, et al., mRNA expression patterns of the IGF system during mouse limb bud development, determined by whole mount in situ hybridization. Mol Cell Endocrinol. 1998 Mar 16;138(1-2):151-61
Assay type: RNA in situ
MGI Accession ID: MGI:6437361
Gene symbol: Igf1r
Gene name: insulin-like growth factor I receptor
Probe: Igf1r cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4G: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: marginal vein of hindlimb Weak 4G
TS19: hindlimb bud mesenchyme Present Regionally restricted 4G Expression was detected in posterior and anterior proximal regions (signal strongest in the anterior region).

Specimen 4H: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hand Present 4H Expression was in the region where the handplate forms.
TS20: hand pre-cartilage condensation Present Regionally restricted 4H Expression was detected in the prechondral skeleton of the digits.

Specimen 4I: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hindlimb phalanx pre-cartilage condensation Present Regionally restricted 4I Expression was detected in the pre-cartilage layers located in the pre-phalangeal joint areas.

Specimen not shown: embryonic day 9.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS15: forelimb bud mesenchyme Present Regionally restricted Expression was localized through the whole mesoderm.

Specimen not shown: embryonic day 10.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted Expression was localized to the proximal mesoderm.

Specimen not shown: embryonic day 11.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted Expression was detected in the anterior and posterior proximal mesoderm, being stronger in the anterior part.
TS19: marginal vein of forelimb Weak

Specimen not shown: embryonic day 13.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS21: forelimb digit pre-cartilage condensation Weak
TS21: forelimb interphalangeal joint Present
TS21: forelimb interdigital region Present Regionally restricted Expression was detected in the proximal interdigital regions.

Specimen not shown: embryonic day 14.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS22: hand cartilage condensation Weak Regionally restricted Expression was seen only in part of the cartilage at the distal part of the hand.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory