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Gene Expression Data
Assay Details
Assay
Reference: J:48168 van Kleffens M, et al., mRNA expression patterns of the IGF system during mouse limb bud development, determined by whole mount in situ hybridization. Mol Cell Endocrinol. 1998 Mar 16;138(1-2):151-61
Assay type: RNA in situ
MGI Accession ID: MGI:6437233
Gene symbol: Igf2
Gene name: insulin-like growth factor 2
Probe: Igf2 cDNA6
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4D: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: hindlimb bud Present Regionally restricted 4D Expression was detected in the anterior and posterior region of the border between foot and leg.
TS19: hindlimb bud mesenchyme Present 4D
TS19: marginal vein of hindlimb Present 4D

Specimen 4E: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hindlimb phalanx pre-cartilage condensation Present Regionally restricted 4E Expression was detected in the separate pre-phalanges.
TS21: marginal vein of hindlimb Weak 4E

Specimen 4F: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: forelimb phalanx cartilage condensation Present Regionally restricted 4F Expression was detected in the pre-phalangeal cells surrounding the pre-phalangeal joint areas.

Specimen not shown: embryonic day 9.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS15: forelimb bud mesenchyme Present Widespread Expression was found in mesodermal cells through the whole limb bud at E9.5.

Specimen not shown: embryonic day 10.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted Expression became limited to the proximal mesoderm forming a ring between the hand and arm.
TS17: marginal vein of forelimb Present

Specimen not shown: embryonic day 11.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted Expression was limited to the proximal mesoderm forming a ring between the hand and arm.
TS19: marginal vein of forelimb Present

Specimen not shown: embryonic day 12.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present Regionally restricted Expression was limited to the proximal mesoderm forming a ring between the hand and arm.
TS20: forelimb digit mesenchyme Present Regionally restricted Expression appears in the first areas where pre-cartilage is formed.

Specimen not shown: embryonic day 13.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS21: forelimb digit pre-cartilage condensation Present


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory