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Gene Expression Data
Assay Details
Assay
Reference: J:48168 van Kleffens M, et al., mRNA expression patterns of the IGF system during mouse limb bud development, determined by whole mount in situ hybridization. Mol Cell Endocrinol. 1998 Mar 16;138(1-2):151-61
Assay type: RNA in situ
MGI Accession ID: MGI:6437203
Gene symbol: Igf1
Gene name: insulin-like growth factor 1
Probe: Igf1 cDNA4
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS16: hindlimb bud mesenchyme Present Regionally restricted 4A Expression was localized primarily in the anterior mesoderm.
Specimen 4B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: hindlimb bud mesenchyme Present Regionally restricted 4B Expression was detected in both anterior and posterior mesodermal cells.
TS19: marginal vein of hindlimb Present 4B
Specimen 4C: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: forelimb Present Regionally restricted 4C Expression was detected at the borders of the pre-cartilage and in a small posterior proximal part.
TS22: forelimb interdigital region Present Regionally restricted 4C Expression was located at the borders of the interdigital region, extending more distally than at E13.5.
Specimen not shown: embryonic day 9.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS15: forelimb bud mesenchyme Strong Regionally restricted Strong signals were found in the anterior mesodermal cells with expression also visible in the posterior mesodermal cells.
Specimen not shown: embryonic day 10.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Strong Regionally restricted Strong signals were found in the anterior mesodermal cells with expression also visible in the posterior mesodermal cells.
Specimen not shown: embryonic day 11.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted Expression in the anterior and posterior mesodermal cells became smaller.
TS19: marginal vein of forelimb Present
Specimen not shown: embryonic day 12.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS20: forelimb interdigital region Present Non-Uniform The interdigital region was stained locally, v-shaped and strongest proximally. Expression started between digit III and IV.
TS20: interdigital region between forelimb digits 3 and 4 Present
Specimen not shown: embryonic day 13.5 (more )
Note: As per schematic overview of expression pattern in Fig. 1.
Structure Level Pattern Image Note
TS21: interdigital region between forelimb digits 2 and 3 Present
TS21: interdigital region between forelimb digits 3 and 4 Present
TS21: interdigital region between forelimb digits 4 and 5 Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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