Antibody:	anti-Eppk1
Detection system:	Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS17: hepatic primordium	Present	Regionally restricted	1A'' , 1A'	Expression overlapping with that of Afp.

Structure	Level	Pattern	Image	Note
TS19: liver	Weak		1B'' , 1B'	Expression decreases at later stages.

Structure	Level	Pattern	Image	Note
TS20: liver	Trace		1C , 1C'' , 1C'''	Expression almost undetectable at E12.5

Structure	Level	Pattern	Image	Note
TS21: liver	Ambiguous	Regionally restricted	1D , 1D''' , 1D''	No expression in hepatoblasts.

Structure	Level	Pattern	Image	Note
TS23: liver	Present	Regionally restricted	2A , 2A'	Expression gradually increasing. Expressing cells located close to smooth muscle cells lining the portal vein.

Structure	Level	Pattern	Image	Note
TS24: intrahepatic bile duct epithelium	Present	Regionally restricted	2B , 2B'	Expression in ductal plate cells near portal vein.

Structure	Level	Pattern	Image	Note
TS23: liver	Present	Regionally restricted	2C' , 2C	Expression gradually increasing. Expressing cells located close to portal vein endothelium.

Structure	Level	Pattern	Image	Note
TS23: liver	Strong	Regionally restricted	2D , 2D''	Strong expression close to portal veins.
TS23: intrahepatic bile duct epithelium	Present	Regionally restricted	2D , 2D''	Expression in cholangiocyte progenitor cells also expressing cytokeratins.
TS23: liver parenchyma	Weak	Regionally restricted	2D , 2D''	Lower level of expression sometimes in parenchyme near portal vein.

Structure	Level	Pattern	Image	Note
TS25: liver	Strong	Regionally restricted	2E'' , 2E	Strong expression close to portal veins.
TS25: intrahepatic bile duct epithelium	Present	Regionally restricted	2E'' , 2E	Expression in cholangiocyte progenitor cells also expressing cytokeratins.
TS25: liver parenchyma	Weak	Regionally restricted	2E , 2E''	Lower level of expression sometimes in parenchyme near portal vein.

Structure	Level	Pattern	Image	Note
TS25: intrahepatic bile duct epithelium	Present	Regionally restricted	S1A , S1A' , S1A''	Expression in cholangiocyte progenitors in forming primitive duct.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Regionally restricted	2F'' , 2F	No expression in hepatocytes.
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	2F , 2F''	Expression in bile duct. Expression in cholangiocyte progenitor cells also expressing cytokeratins.

Structure	Level	Pattern	Image	Note
TS23: liver	Strong	Regionally restricted	2G	Strong expression close to portal veins. No expression in hepatocytes.
TS23: intrahepatic bile duct epithelium	Present		2G	Expression in primary duct.

Structure	Level	Pattern	Image	Note
TS25: liver	Strong	Regionally restricted	2H	Strong expression close to portal veins. No expression in hepatocytes.
TS25: intrahepatic bile duct epithelium	Present		2H	Expression in primary duct.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Regionally restricted	2I	No expression in hepatocytes.
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	2I	Expression in primary duct.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Regionally restricted	3A	Expression in hepatic progenitor cells.
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	3A	Expression in cholangiocytes near the portal vein.

Structure	Level	Pattern	Image	Note
TS28: liver lobule	Absent		3A1' , 3A1''

Structure	Level	Pattern	Image	Note
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	3A2'' , 3A2'	Expressing cells near the portal vein.

Structure	Level	Pattern	Image	Note
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	3C' , 3C''' , 3C	Co-expression with A6 antigen in cholangiocytes.

Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Ambiguous	Regionally restricted	S2A	Low or undetectable levels of expression in most hepatocytes

Structure	Level	Pattern	Image	Note
TS28: intrahepatic bile duct epithelium	Strong	Regionally restricted	S2A'	High level of expression in cholangiocytes.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Regionally restricted	S2A''	Expression in hepatocytes close to portal vein.

Structure	Level	Pattern	Image	Note
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	4A	Co-expression with cytokeratins in cholangiocyte progenitors.

Structure	Level	Pattern	Image	Note
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	4C	Co-expression with Cdh1 in cholangiocyte progenitors.

Structure	Level	Pattern	Image	Note
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	4E , 4E''	Co-expression with Opn (Spp1) in cholangiocyte progenitors.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Regionally restricted	4G , 4G'	No co-expression with Pcna in normal liver.

Structure	Level	Pattern	Image	Note
TS28: intrahepatic bile duct epithelium	Present	Regionally restricted	4I' , 4I	Co-expression with Ctnnb1 in bile duct.