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Gene Expression Data
Assay Details
Assay
Reference: J:139183 Smagulova FO, et al., GATA4/FOG2 transcriptional complex regulates Lhx9 gene expression in murine heart development. BMC Dev Biol. 2008;8:67
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6434391
Gene symbol: Lhx9
Gene name: LIM homeobox protein 9
Antibody: anti-LH2A/LH2B
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 4A/C: embryonic day 11.5 (more )
Note: Double labeled: red - Lhx9; green - Tnnt2 (assay MGI:6434396).
Structure Level Pattern Image Note
TS19: cardiac muscle tissue Weak 4C , 4A
TS19: heart Strong Regionally restricted 4A , 4C Localized to the outmost cardiac layer, not co-localized with cardiac muscle marker Tnnt2.
Specimen 4D/F: embryonic day 11.5 (more )
Note: Double labeled: red - Lhx9; green - Eng (assay MGI:6434395).
Structure Level Pattern Image Note
TS19: visceral pericardium Present 4F , 4D Not co-localized with endothelial lineage marker Eng.
TS19: cardiac muscle tissue Weak 4F , 4D
Specimen 4G/I: embryonic day 12.5 (more )
Note: Double labeled: red - Lhx9; green - Eng (assay MGI:6434395).
Structure Level Pattern Image Note
TS20: visceral pericardium Present 4I , 4G Some (but not all) ventricular cells in the epicardial layer coexpressed Eng.
TS20: heart mesenchyme Present Regionally restricted 4I , 4G Some (but not all) cells in the sub-epicardial layer coexpressed Eng.
TS20: cardiac muscle tissue Weak 4I , 4G
Specimen 4J/L: embryonic day 12.5; Zfpm2tm1Sho/Zfpm2tm1Sho (more )
Note: Double labeled: red - Lhx9; green - Eng (assay MGI:6434395).
Structure Level Pattern Image Note
TS20: visceral pericardium Present 4J , 4L Some (but not all) ventricular cells in the epicardial layer coexpressed Eng.
TS20: heart mesenchyme Present Regionally restricted 4L , 4J Some (but not all) cells in the sub-epicardial layer coexpressed Eng.
TS20: cardiac muscle tissue Weak 4L , 4J
Specimen 8: embryonic day 10.5 (more )
Note: Double labeled: red - Lhx9; green - Isl1 (assay MGI:6434397).
Structure Level Pattern Image Note
TS17: liver Present 8_lower right , 8_upper right , 8_lower left , 8_upper left Coexpressed with Isl1.
TS17: septum transversum Present 8_upper right , 8_upper left
TS17: visceral pericardium Present 8_upper right , 8_upper left
Specimen S2C: embryonic day 11.5; Zfpm2tm1Sho/Zfpm2tm1Sho (more )
Structure Level Pattern Image Note
TS19: gonad Strong S2C
Specimen not shown: embryonic day 12.5 (more )
Note: Also stained for Tnnt2 (assay MGI:6434396).
Structure Level Pattern Image Note
TS20: heart Strong Regionally restricted Localized to the outmost cardiac layer, not co-localized with cardiac muscle marker Tnnt2.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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