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Gene Expression Data
Assay Details
Assay
Reference: J:268201 Zhang Z, et al., Generation of MLC-2v-tdTomato knock-in reporter mouse line. Genesis. 2018 Oct;56(10):e23256
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6433045
Gene symbol: Myl2
Gene name: myosin, light polypeptide 2, regulatory, cardiac, slow
Reporter: tdTomato
Direct Detection
Results
Specimen 2a: embryonic day 8.0; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS12: primitive ventricle Present 2a Merged , 2a Red Fluorescence
Specimen 2b: embryonic day 8.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS13: primitive ventricle Present 2b Merged , 2b Red Fluorescence
Specimen 2c: embryonic day 9.0; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS14: primitive ventricle Present 2c Red Fluorescence , 2c Merged
Specimen 2d: embryonic day 10.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS17: primitive ventricle Present 2d Merged , 2d Red Fluorescence
Specimen 2e: embryonic day 13.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS21: heart ventricle Present 2e Red Fluorescence , 2e Merged Ventricular-specific expression pattern was observed at E13.5. Other areas outside the heart did not show tdTomato expression.
TS21: heart atrium Absent 2e Merged , 2e Red Fluorescence
Specimen 2f: embryonic day 16.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS24: heart ventricle Present 2f Red Fluorescence , 2f Merged Ventricular-specific expression pattern was observed at E16.5. Other areas outside the heart did not show tdTomato expression.
TS24: heart atrium Absent 2f Red Fluorescence , 2f Merged
Specimen 2g: embryonic day 9.0; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS14: primitive ventricle Present 2g Merged , 2g Red Fluorescence
TS14: outflow tract Absent 2g Red Fluorescence , 2g Merged
TS14: sinus venosus Absent 2g Red Fluorescence , 2g Merged
Specimen 2h: embryonic day 10.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS17: primitive ventricle Present 2h Red Fluorescence , 2h Merged
Specimen 2i: embryonic day 13.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS21: heart ventricle Present 2i Merged , 2i Red Fluorescence Ventricular-specific expression pattern was observed at E13.5.
TS21: heart atrium Absent 2i Red Fluorescence , 2i Merged
Specimen 2j: embryonic day 16.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS24: heart ventricle Present 2j Merged , 2j Red Fluorescence Ventricular-specific expression pattern was observed at E16.5.
TS24: heart atrium Absent 2j Red Fluorescence , 2j Merged
Specimen 3a: embryonic day 10.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Double labeled: red - Myl2; green - alpha-actinin, a cardiomyocyte marker.
Structure Level Pattern Image Note
TS17: primitive ventricle Present 3e tdTomato DAPI , 3a tdTomato DAPI , 3b Merged , 3e Merged , 3a Merged , 3b tdTomato DAPI tdTomato was expressed throughout the entire ventricle. Only the heart in the entire chest area showed expression. Every cardiomyocyte in the ventricle expressing alpha-actinin showed tdTomato label.
Specimen 3c: embryonic day 13.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Double labeled: red - Myl2; green - alpha-actinin, a cardiomyocyte marker.
Structure Level Pattern Image Note
TS21: heart ventricle Present 3c tdTomato DAPI , 3f tdTomato DAPI , 3f Merged , 3c Merged tdTomato was expressed throughout the entire ventricle. Every cardiomyocyte in the ventricle expressing alpha-actinin showed tdTomato label.
TS21: heart left ventricle Present Uniform 3f tdTomato DAPI , 3f Merged , 3c Merged , 3c tdTomato DAPI
TS21: heart right ventricle Present Uniform 3f tdTomato DAPI , 3f Merged , 3c Merged , 3c tdTomato DAPI
Specimen 3d: embryonic day 16.5; Myl2tm1.1Cya/Myl2+ (more )
Note: Double labeled: red - Myl2; green - alpha-actinin, a cardiomyocyte marker.
Structure Level Pattern Image Note
TS24: heart ventricle Present 3d tdTomato DAPI , 3g tdTomato DAPI , 3g Merged , 3d Merged tdTomato was expressed throughout the entire ventricle. Every cardiomyocyte in the ventricle expressing alpha-actinin showed tdTomato label.
TS24: heart left ventricle Present Uniform 3g Merged , 3g tdTomato DAPI , 3d Merged , 3d tdTomato DAPI
TS24: heart right ventricle Present Uniform 3g Merged , 3g tdTomato DAPI , 3d Merged , 3d tdTomato DAPI
Specimen 4a P1: postnatal day 1; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS27: heart ventricle Present 4a P1 Merged , 4a P1 Red Fluorescence
Specimen 4a 6wk: postnatal week 6; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS28: heart ventricle Present 4a 6wk Merged , 4a 6wk Red Fluorescence
Specimen 4b +/+: postnatal day 0; Myl2tm1.1Cya/Myl2tm1.1Cya (more )
Note: Triple labeled: red - Myl2; yellow - Myl2 (assay MGI:6433831); green - Tnnt2 (assay MGI:6433836).
Structure Level Pattern Image Note
TS27: heart ventricle Present 4b +/+ Merged , 4b +/+ tdTomato
Specimen 4b +/-: postnatal day 0; Myl2tm1.1Cya/Myl2+ (more )
Note: Triple labeled: red - Myl2; yellow - Myl2 (assay MGI:6433831); green - Tnnt2 (assay MGI:6433836).
Structure Level Pattern Image Note
TS27: heart ventricle Present 4b +/- Merged , 4b +/- tdTomato
Specimen 4c +/+: postnatal week 6; Myl2tm1.1Cya/Myl2tm1.1Cya (more )
Note: Double labeled: red - Myl2; yellow - Myl2 (assay MGI:6433831).
Structure Level Pattern Image Note
TS28: heart ventricle Strong 4c +/+ Merged top , 4c +/+ tdTomato bottom , 4c +/+ Merged bottom , 4c +/+ tdTomato top
Specimen 5a E20 E: embryonic day 20.0; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS26: extensor digitorum longus Absent 5a E20 Red Fluorescence E , 5a E20 Merged E Expression was not detected in fast twitch muscle.
Specimen 5a E20 S: embryonic day 20.0; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS26: soleus Absent 5a E20 Red Fluorescence S , 5a E20 Merged S
Specimen 5a P0 E: postnatal day 0; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS27: extensor digitorum longus Absent 5a P0 Red Fluorescence E , 5a P0 Merged E Expression was not detected in fast twitch muscle.
Specimen 5a P0 S: postnatal day 0; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS27: soleus Present 5a P0 Red Fluorescence S , 5a P0 Merged S Expression in slow twitch muscle first appeared at P0.
Specimen 5a P1 E: postnatal day 1; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS27: extensor digitorum longus Absent 5a P1 Red Fluorescence E , 5a P1 Merged E Expression was not detected in fast twitch muscle.
Specimen 5a P1 S: postnatal day 1; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS27: soleus Present 5a P1 Red Fluorescence S , 5a P1 Merged S
Specimen 5a 6wk E: postnatal week 6; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS28: extensor digitorum longus Absent 5a 6wk Merged E , 5a 6wk Red Fluorescence E Expression was not detected in fast twitch muscle.
Specimen 5a 6wk S: postnatal week 6; Myl2tm1.1Cya/Myl2+ (more )
Note: Merged image is Bright Field combined with Red Fluorescence image.
Structure Level Pattern Image Note
TS28: soleus Present 5a 6wk Merged S , 5a 6wk Red Fluorescence S Expression in slow twitch muscle was significantly increased in the adult compared with the muscle at P0 or P1.
Specimen 5b S left: postnatal day 1; Myl2tm1.1Cya/Myl2+ (more )
Note: Triple labeled: red - Myl2 reporter; green - Acta1 (assay MGI:6434003); yellow - slow muscle myosin.
Structure Level Pattern Image Note
TS27: soleus Present 5b S Merged left , 5b S tdTomato left Expression of the reporter in slow twitch muscle overlapped with a slow muscle myosin.
Specimen 5b S right: postnatal day 1; Myl2tm1.1Cya/Myl2+ (more )
Note: Triple labeled: red - Myl2 reporter; green - Acta1 (assay MGI:6434003); yellow - fast muscle myosin.
Structure Level Pattern Image Note
TS27: soleus Present 5b S Merged right , 5b S tdTomato right Expression of the reporter in slow twitch muscle did not colocalize with a fast muscle myosin.
Specimen 5b E left: postnatal day 1; Myl2tm1.1Cya/Myl2+ (more )
Note: Triple labeled: red - Myl2 reporter; green - Acta1 (assay MGI:6434003); yellow - slow muscle myosin.
Structure Level Pattern Image Note
TS27: extensor digitorum longus Absent 5b E tdTomato left , 5b E Merged left The reporter was not expressed in fast twitch muscle.
Specimen 5b E right: postnatal day 1; Myl2tm1.1Cya/Myl2+ (more )
Note: Triple labeled: red - Myl2 reporter; green - Acta1 (assay MGI:6434003); yellow - fast muscle myosin.
Structure Level Pattern Image Note
TS27: extensor digitorum longus Absent 5b E tdTomato right , 5b E Merged right The reporter was not expressed in fast twitch muscle.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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