Pcdh8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:98484 Lickert H, et al., Dissecting Wnt/{beta}-catenin signaling during gastrulation using RNA interference in mouse embryos. Development. 2005 Jun;132(11):2599-609
Assay type:	RNA in situ
MGI Accession ID:	MGI:6432919
Gene symbol:	Pcdh8
Gene name:	protocadherin 8

Probe:	PAPC probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 6E PAPC wt (upper): embryonic day 8.0; Ctnnb1^tm2Kem/Ctnnb1^tm2.1Kem (more

)
Note: 2-3 somite stage.

Structure	Level	Pattern	Image	Note
TS12: unsegmented mesenchyme	Present	Regionally restricted	6E PAPC wt (upper)	Two stripes of expression at anterior end of presomitic mesoderm corresponding to the next 2 presumptive somites.

Specimen 6E PAPC wt (lower): embryonic day 8.5; Ctnnb1^tm2Kem/Ctnnb1^tm2.1Kem (more

)
Note: 9-10 somite stage.

Structure	Level	Pattern	Image	Note
TS13: unsegmented mesenchyme	Present	Regionally restricted	6E PAPC wt (lower)	Two stripes of expression at anterior end of presomitic mesoderm corresponding to the next 2 presumptive somites.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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