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Gene Expression Data
Assay Details
Assay
Reference: J:102943 Demyanenko GP, et al., Abnormal neocortical development in mice lacking cGMP-dependent protein kinase I. Brain Res Dev Brain Res. 2005 Nov 7;160(1):1-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6432399
Gene symbol: Prkg1
Gene name: protein kinase, cGMP-dependent, type I
Antibody: anti-cGKI
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex ventricular layer Present 1A Expressed in radially oriented processes.
Specimen 1B/D: embryonic day 14.5 (more )
Note: Double labeled: green - Prkg1; red - Nes (assay MGI:6432400).
Structure Level Pattern Image Note
TS22: cerebral cortex marginal layer Present 1B , 1D Most prominent expression. Expressed in radially oriented processes. Colocalized with Nes in radial glial processes.
TS22: cerebral cortex subventricular zone Present 1B , 1D Most prominent expression. Expressed in radially oriented processes. Colocalized with Nes in radial glial processes.
TS22: cerebral cortex ventricular layer Present 1B , 1D Most prominent expression. Expressed in radially oriented processes. Colocalized with Nes in radial glial processes.
TS22: cortical plate Present 1B , 1D Most prominent expression. Expressed in radially oriented processes. Colocalized with Nes in radial glial processes.
TS22: cerebral cortex intermediate zone Strong 1D , 1B Most prominent expression. Expressed in radially oriented processes. Colocalized with Nes in radial glial processes. Strong expression in tangentially oriented axonal processes.
Specimen 1E: embryonic day 14.5 (more )
Note: Double labeled: green - Prkg1; red - Nes (assay MGI:6432400).
Structure Level Pattern Image Note
TS22: cerebral cortex marginal layer Present 1E Colocalized with Nes in radial glial processes. Also expressed in cells that were not Nes positive.
Specimen 1F: embryonic day 14.5 (more )
Note: Double labeled: green - Prkg1; red - Nes (assay MGI:6432400).
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present 1F Colocalized with Nes in radial glial processes. Also expressed in cells that were not Nes positive.
Specimen 1G: embryonic day 14.5; Prkg1tm1Hfm/Prkg1tm1Hfm (more )
Structure Level Pattern Image Note
TS22: cerebral cortex Absent 1G
Specimen 1H/J: embryonic day 14.5 (more )
Note: Double labeled: green - Prkg1; red - Tubb3 (assay MGI:6432401).
Structure Level Pattern Image Note
TS22: cortical plate Present 1H , 1J Expressed in Tubb3+ neurons.
TS22: cerebral cortex intermediate zone Present Regionally restricted 1H , 1J Expressed in Tubb3+ neurons in the upper region.
Specimen 1K: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: cerebral cortex intermediate zone Present 1K Decreased expression.
TS25: cerebral cortex marginal zone Present 1K Decreased expression.
TS25: cerebral cortex subventricular zone Present 1K Decreased expression.
TS25: cerebral cortex ventricular layer Present 1K Decreased expression.
TS25: cortical plate Present 1K Decreased expression.
Specimen 1L: postnatal day 28 (more )
Structure Level Pattern Image Note
TS28: neocortex Trace 1L
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory