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Gene Expression Data
Assay Details
Assay
Reference: J:216551 Ramasamy S, et al., NogoR1 and PirB signaling stimulates neural stem cell survival and proliferation. Stem Cells. 2014 Jun;32(6):1636-48
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6431318
Gene symbol: Nes
Gene name: nestin
Antibody: Anti-Nestin
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2Ab: embryonic day 14.5 (more )
Note: Double labeled: green - Nes; red - Rtn4r (assay MGI:6431322).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Present 2Ad , 2Af , 2Ab , 2Ah Expression co-localized with Rtn4r.
TS22: cerebral cortex ventricular layer Present 2Ad , 2Af , 2Ab , 2Ah Expression co-localized with Rtn4r.
TS22: ganglionic eminence Present 2Aj , 2Al , 2Ab , 2Ad Expression co-localized with Rtn4r.
Specimen 2Bb: embryonic day 14.5 (more )
Note: Double labeled: green - Nes; red - Pirb (assay MGI:6431326).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Present 2Bb , 2Bd , 2Bh , 2Bf Expression co-localized with Pirb.
TS22: cerebral cortex ventricular layer Present 2Bb , 2Bd , 2Bh , 2Bf Expression co-localized with Pirb.
TS22: ganglionic eminence Present 2Bd , 2Bb , 2Bl , 2Bj Expression co-localized with Pirb.
Specimen 3A: embryonic day 15.5 (more )
Note: Triple labeled: green - Nes; blue - Rtn4r (assay MGI:6431322); red - Tubb3 (assay MGI:6431327).
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present Regionally restricted 3A Merged , 3A Nestin Labeling is specific to the apical ventricular zone/subventricular zone of the dorsal cortex. Expression did not colocalize with Tubb3 or Rtn4r.
TS23: cerebral cortex ventricular layer Present Regionally restricted 3A Merged , 3A Nestin Labeling is specific to the apical ventricular zone/subventricular zone of the dorsal cortex. Expression did not colocalize with Tubb3 or Rtn4r.
Specimen 3B: embryonic day 15.5 (more )
Note: Triple labeled: green - Nes; blue - Pirb (assay MGI:6431326); red - Tubb3 (assay MGI:6431327).
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present Regionally restricted 3B Nestin , 3B Merged Labeling is specific to the apical ventricular zone/subventricular zone of the dorsal cortex. Expression did not colocalize with Tubb3 or Pirb.
TS23: cerebral cortex ventricular layer Present Regionally restricted 3B Nestin , 3B Merged Labeling is specific to the apical ventricular zone/subventricular zone of the dorsal cortex. Expression did not colocalize with Tubb3 or Pirb.
Specimen S1A: embryonic day 14.5; Pirbtm1Ttk/Pirbtm1Ttk (more )
Note: Two Pirb alleles were used in the publication, but the one in this assay was not specified; it could have been either Pirbtm1Stz or Pirbtm1Ttk. Double labeled: green - Nes; red - Pirb (assay MGI:6431326).
Structure Level Pattern Image Note
TS22: brain Present S1A Merged , S1A Nestin
Specimen S1B: embryonic day 14.5; Rtn4rtm1Matl/Rtn4rtm1Matl (more )
Note: Double labeled: green - Nes; red - Rtn4r (assay MGI:6431322).
Structure Level Pattern Image Note
TS22: brain Present S1B Nestin , S1B Merged
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory