Antibody:	Anti-Shh
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: lung epithelium	Present		1C , 1D

Structure	Level	Pattern	Image	Note
TS19: lung epithelium	Present	Regionally restricted	2A , 2B	Expression was detected in epithelial cells of the primordial tubules.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present		2C , 2D	Expression was detected throughout the pulmonary epithelium at E12.5.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present	Regionally restricted	2F , 2E	Expression was restricted to epithelial cells in the distal region of the primordial tubules on E13.5. The proximal region was devoid of staining.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present	Regionally restricted	3B , 3A	Expression was detected in the respiratory epithelium of the distal acinar tubules. The proximal region was devoid of staining.

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present	Regionally restricted	3C , 3D	Expression was detected in the respiratory epithelium of the distal acinar tubules. The proximal region was devoid of staining.

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Present	Regionally restricted	3F , 3E	Expression was detected primarily in the epithelium of the acinar tubules.
TS24: bronchiole epithelium	Weak	Regionally restricted	3E , 3F	Some staining was seen in the conducting epithelium of the bronchiolar tubules. Localization of staining was consistent with non-ciliated epithelial cells.

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present		4A
TS25: bronchiole epithelium	Strong		4A	Localization of staining was consistent with non-ciliated epithelial cells. Intensity of staining peaked on E17.0/17.5.
TS25: bronchus epithelium	Strong		4A	Localization of staining was consistent with non-ciliated epithelial cells. Intensity of staining peaked on E17.0/17.5.

Structure	Level	Pattern	Image	Note
TS25: bronchus epithelium	Strong		4B	Intensity of staining peaked on E17.0/17.5.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present	Regionally restricted	4C	Expression was detected in both respiratory and conducting airways. Expression remained intense in the distal respiratory epithelium (i.e. terminal saccules).
TS26: bronchiole epithelium	Strong		4C	The peak expression level was maintained at E18.0.
TS26: bronchus epithelium	Strong		4C	The peak expression level was maintained at E18.0.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		4D	Expression was detected in both respiratory and conducting airways.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		4E	Expression was detected in both respiratory and conducting airways.

Structure	Level	Pattern	Image	Note
TS27: lung epithelium	Weak		4F	Expression was detected in the respiratory epithelium, where it was markedly reduced.
TS27: bronchiole epithelium	Strong		4F

Structure	Level	Pattern	Image	Note
TS27: bronchiole epithelium	Strong		4G

Structure	Level	Pattern	Image	Note
TS28: alveolus epithelium	Weak

Structure	Level	Pattern	Image	Note
TS28: bronchus epithelium	Present

Structure	Level	Pattern	Image	Note
TS28: alveolus epithelium	Absent
TS28: bronchus epithelium	Absent

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present	Regionally restricted	5A	Expression was detected in the peripheral respiratory epithelium.
TS26: bronchiole epithelium	Present		5A

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present	Regionally restricted	6A	Expression was detected in the peripheral respiratory airway epithelial cells.
TS26: bronchiole epithelium	Present	Regionally restricted	6A	Expression was detected in dome-shaped, protuding cells, consistent with non-ciliated Clara cells.

Structure	Level	Pattern	Image	Note
TS26: bronchiole epithelium	Present	Regionally restricted	6C	Expression was detected in dome-shaped, protuding cells, consistent with non-ciliated Clara cells.

Structure	Level	Pattern	Image	Note
TS26: bronchiole epithelium	Present	Regionally restricted	6E	Expression was detected in dome-shaped, protuding cells, consistent with non-ciliated Clara cells.