Hoxb1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:173839 Bertrand N, et al., Hox genes define distinct progenitor sub-domains within the second heart field. Dev Biol. 2011 May 15;353(2):266-74
Assay type:	RNA in situ
MGI Accession ID:	MGI:6430423
Gene symbol:	Hoxb1
Gene name:	homeobox B1

Probe:	Hoxb1 probe29
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

S1G ©

Specimen S1G: embryonic day 7.25 (more

)

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		S1G	Transcription initiates earlier than Hoxa1 and Hoxa3.

S1H ©

Specimen S1H: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	S1H	Authors report expansion of expression in anterior lateral mesoderm.

S1I ©

Specimen S1I: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: embryo mesoderm	Present	Regionally restricted	S1I	Authors report expansion of expression in anterior lateral mesoderm.

1A ©

Specimen 1A: embryonic day 7.75 (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo mesoderm	Present	Regionally restricted	1A	Most anterior border of expression near cardiac crescent, more rostral than those of Hoxa1 and Hoxa3.

2A ©

Specimen 2A: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	2A	Expression in ventral and lateral foregut endoderm.
TS13: splanchnopleure	Present		2A	Expression in splanchnic mesoderm.
TS13: endocardial tube	Absent		2A	No expression in heart tube.

2A1 ©

Specimen 2A1: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	2A1	Expression in anterior foregut endoderm.

2A2 ©

Specimen 2A2: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	2A2	Expression in anterior foregut endoderm.
TS13: secondary heart field	Present	Regionally restricted	2A2	Expression in medial domain of second heart field.

2A3 ©

Specimen 2A3: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	2A3	Expression in anterior foregut endoderm.
TS13: secondary heart field	Present	Regionally restricted	2A3	Expression in posterior domain of second heart field.
TS13: endocardial tube	Absent		2A3	No expression in heart tube.

S4F ©

Specimen S4F: embryonic day 7.25 (more

)

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		S4F

S4G ©

Specimen S4G: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: secondary heart field	Present	Regionally restricted	S4G	Anterior expansion of expression in second heart field.

S4H ©

Specimen S4H: embryonic day 7.75 (more

)

Structure	Level	Pattern	Image	Note
TS12: secondary heart field	Present	Regionally restricted	S4H	Anterior expansion of expression in second heart field.

S4I ©

Specimen S4I: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: endocardial tube	Absent		S4I

S4J ©

Specimen S4J: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: endocardial tube	Absent		S4J

S6A ©

Specimen S6A: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present	Regionally restricted	S6A	Expression in splanchnic mesoderm situated posterior and adjacent to cardiac tube.

S6A inset ©

Specimen S6A inset: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present	Regionally restricted	S6A inset	Expression in splanchnic mesoderm situated posterior and adjacent to cardiac tube.

S6B ©

Specimen S6B: embryonic day 8.5; Aldh1a2^tm1Ipc/Aldh1a2^tm1Ipc (more

)

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Weak	Regionally restricted	S6B	Expression downregulated in splanchnic mesoderm in mutant embryos.

S6B inset ©

Specimen S6B inset: embryonic day 8.5; Aldh1a2^tm1Ipc/Aldh1a2^tm1Ipc (more

)

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Weak	Regionally restricted	S6B inset	Expression downregulated in splanchnic mesoderm in mutant embryos.

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