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Gene Expression Data
Assay Details
Assay
Reference: J:181884 Quinn ME, et al., Preaxial polydactyly caused by Gli3 haploinsufficiency is rescued by Zic3 loss of function in mice. Hum Mol Genet. 2012 Apr 15;21(8):1888-96
Assay type: Western blot
MGI Accession ID: MGI:6430387
Gene symbol: Gli3
Gene name: GLI-Kruppel family member GLI3
Antibody: Gli3ab1
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Quantification and ratio of protein levels are shown in 6B and 6C.
Results Image: 6A
 Sample Information Bands Other Sample Information
Lane AgeStructure 190 kDa (a) 83 kDa (b) 66 kDa (c) Genetic BackgroundMutant Allele(s)Sex Note
WT A E11.5 TS19: forelimb bud Present Strong Absent involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Not Specified Anterior half of the forelimb.
WT P E11.5 TS19: forelimb bud Present Present Absent involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Not Specified Posterior half of the forelimb bud.
Gli3+/- A E11.5 TS19: forelimb bud Present Strong Present involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3+Not Specified Anterior half of the forelimb.
Gli3+/- P E11.5 TS19: forelimb bud Present Present Present involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3+Not Specified Posterior half of the forelimb.
Gli3+/- A E11.5 TS19: forelimb bud Present Strong Present involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3+Not Specified Anterior half of the forelimb.
Gli3+/- P E11.5 TS19: forelimb bud Present Present Present involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3+Not Specified Posterior half of the forelimb.
Zic3 null;Gli3+/- E11.5 TS19: forelimb bud Present Strong Present involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3+, Zic3tm1Bca/Zic3tm1BcaNot Specified Anterior half of the forelimb.
Zic3 null;Gli3+/- E11.5 TS19: forelimb bud Present Present Present involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3+, Zic3tm1Bca/Zic3tm1BcaNot Specified Posterior half of the forelimb bud.
Notes:
(a) This band represents the full-length protein.
(b) This band represents the repressor protein.
(c) This band represents the truncated mutant Gli3 protein (Xt-J).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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