Gene Expression Data
Assay Details
Assay
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Reference:
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J:287389
Hughes JH, et al., Conditional targeting in mice reveals that hepatic homogentisate 1,2-dioxygenase activity is essential in reducing circulating homogentisic acid and for effective therapy in the genetic disease alkaptonuria. Hum Mol Genet. 2019 Dec 1;28(23):3928-3939
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6423321
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Gene symbol:
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Hgd
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Gene name:
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homogentisate 1, 2-dioxygenase
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
3A Hgd tm1a -/- 2 hr (upper):
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
3A Hgd tm1a -/- Overnight (upper):
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
3A Hgd tm1a -/- 2 hr (lower):
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
3A Hgd tm1a -/- Overnight (lower):
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
heterozygous (not shown):
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgd+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
all other tissues (not shown):
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
3C:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: liver parenchyma |
Present |
Widespread |
3C
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Expression in cytoplasm throughout liver parenchyma.
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Specimen
3D:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: renal cortex |
Present |
Regionally restricted |
3D
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Expression only in certain tubules.
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Specimen
3E:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
3F:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
3G E13.5:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
3G E14.5:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS22: liver |
Present |
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3G E14.5
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Expression in liver from E14.5 onwards.
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Specimen
3G E15.5:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS23: liver |
Present |
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3G E15.5
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Expression in liver from E14.5 onwards.
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Specimen
3H E11.5 Liver:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
3H E12.5 Liver:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
3H E13.5 Liver:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
3H E13.5 Kidney:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
3H E14.5 Kidney:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
3H E15.5 Kidney:
(close )
Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Hgdtm1a(KOMP)Wtsi/Hgdtm1a(KOMP)Wtsi
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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