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Gene Expression Data
Assay Details
Assay
Reference: J:272566 Yang R, et al., CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development. Development. 2019 Jan 25;146(2):dev169235
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6423288
Gene symbol: Cdk5rap3
Gene name: CDK5 regulatory subunit associated protein 3
Antibody: Anti-CDK5RAP3 (H-300; sc-134627)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were described MGI:6423118 and MGI:6423120, but the one used in this assay was not specified. Either antibody could have been used.
Results
Specimen 2B/D: embryonic day 16.5 (more )
Note: Double labeled: green - Cdk5rap3; red - Dlk1 (assay MGI:6423291).
Structure Level Pattern Image Note
TS24: liver Present Regionally restricted 2D , 2B Expression was detected in hepatocytes colocalized with Dlk1 staining.
Specimen 2B'/D': embryonic day 16.5; Cdk5rap3tm1b(EUCOMM)Hmgu/Cdk5rap3tm1b(EUCOMM)Hmgu (more )
Note: Double labeled: green - Cdk5rap3; red - Dlk1 (assay MGI:6423291).
Structure Level Pattern Image Note
TS24: liver Ambiguous 2B' , 2D'
Specimen S7D: postnatal month 1 (more )
Note: Double labeled: green - Cdk5rap3; red - P4hb (assay MGI:6423447).
Structure Level Pattern Image Note
TS28: liver Present S7D bottom , S7D top Expression of Cdk5rap3 colocalized with P4hb, an endoplasmic reticulum marker.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory