Gene Expression Data
Assay Details
Assay
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Reference:
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J:74072
Niinobe M, et al., Cellular and subcellular localization of necdin in fetal and adult mouse brain. Dev Neurosci. 2000;22(4):310-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6423257
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Gene symbol:
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Ndn
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Gene name:
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necdin, MAGE family member
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Antibody:
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anti-necdin
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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|
Results
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Specimen
4A:
embryonic day 15.0
(more )
Note: Fixative was 2% paraformaldehyde/1% picric acid.
Specimen
4A:
(close )
Genetic Background:
|
DDY
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
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Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Fixative was 2% paraformaldehyde/1% picric acid.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: brain nucleus |
Strong |
Regionally restricted |
4A
|
Expression is in the dosomedial hypothalamic nucleus. Expression is in the perikaryon and some process-like structures.
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TS23: hypothalamus |
Strong |
Regionally restricted |
4A
|
Expression is in the dosomedial hypothalamic nucleus. Expression is in the perikaryon and some process-like structures.
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Specimen
4B:
embryonic day 15.0
(more )
Note: Fixative was 2% paraformaldehyde/1% picric acid.
Specimen
4B:
(close )
Genetic Background:
|
DDY
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Fixative was 2% paraformaldehyde/1% picric acid.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: basal ganglia |
Strong |
Regionally restricted |
4B
|
Expression is in the lateral septal nucleus of the basal ganglia. Expression is in the perikaryon and some process-like structures.
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TS23: brain nucleus |
Strong |
Regionally restricted |
4B
|
Expression is in the lateral septal nucleus of the basal ganglia. Expression is in the perikaryon and some process-like structures.
|
Specimen
4C:
embryonic day 15.0
(more )
Note: Fixative was 2% paraformaldehyde/1% picric acid.
Specimen
4C:
(close )
Genetic Background:
|
DDY
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Fixative was 2% paraformaldehyde/1% picric acid.
|
Specimen
4D:
embryonic day 15.0
(more )
Note: Fixative was 2% paraformaldehyde/1% picric acid.
Specimen
4D:
(close )
Genetic Background:
|
DDY
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Fixative was 2% paraformaldehyde/1% picric acid.
|
Specimen
4E:
embryonic day 15.0
(more )
Note: Fixative was 2% paraformaldehyde/1% picric acid.
Specimen
4E:
(close )
Genetic Background:
|
DDY
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Fixative was 2% paraformaldehyde/1% picric acid.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: midbrain tegmentum |
Weak |
Regionally restricted |
4E
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Expression is in the nucleus raphe dorsalis.
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Specimen
4F:
embryonic day 15.0
(more )
Note: Fixative was 2% paraformaldehyde/1% picric acid.
Specimen
4F:
(close )
Genetic Background:
|
DDY
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Fixative was 2% paraformaldehyde/1% picric acid.
|
Specimen
5A:
postnatal day 70
(more )
Specimen
5A:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal day 70
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5C:
postnatal day 70
(more )
Specimen
5C:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal day 70
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: frontal cortex |
Moderate |
|
5C
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Most of the staining is cytoplasmic.
|
Specimen
5D:
postnatal day 70
(more )
Specimen
5D:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal day 70
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Trace |
|
5D
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There is little or no immunoreactivity.
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Specimen
5E:
postnatal day 70
(more )
Specimen
5E:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal day 70
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: substantia nigra |
Present |
Single cells |
5E
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Most of the staining is cytoplasmic.
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Specimen
5F:
postnatal day 70
(more )
Specimen
5F:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal day 70
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|