Antibody:	srPLP/srDM20 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Strong		2A	Cell bodies of dorsal root ganglion neurons were stained intensely.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Strong	Regionally restricted	2C , 2D	Strong immunostaining was detected in motor neurons of the lateral column in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS21: cortical preplate	Present		3A
TS21: cerebral cortex ventricular layer	Present		3A	Expression was detected in the dividing and early post-mitotic cells of the ventricular zone.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex marginal zone	Present		3B
TS25: cerebral cortex subventricular zone	Present		3B
TS25: cortical plate	Present		3B
TS25: cortical subplate	Present		3B
TS25: cerebral cortex intermediate zone	Present		3B	Strings of immunopositive cells were detected throughout the intermediate zone, which likely represent migrating glia and/or neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3C	Expression was detected in all cortical layers at P7.
TS28: cortical layer V	Strong		3C
TS28: cortical layer VI	Strong		3C	Stronger staining was noted in layer VIb.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		3D	Expression was restricted to pyramidal neurons.
TS28: cortical layer III	Present		3D	Expression was restricted to pyramidal neurons.
TS28: cortical layer V	Present		3D	Expression was restricted to pyramidal neurons.
TS28: cortical layer VI	Present		3D	Expression was restricted to pyramidal neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Present			Expression in the adult was considerable reduced; however, immunoreactivity persisted in layers V and VIb.
TS28: cortical layer VI	Present			Expression in the adult was considerable reduced; however, immunoreactivity persisted in layers V and VIb.
TS28: anterior olfactory nucleus	Present
TS28: cerebellum Purkinje cell layer	Present
TS28: cerebellum internal granule cell layer	Present

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong		4A	Strong immunoreactivity was detected in the periglomerular cells and in the glomeruli.
TS28: main olfactory bulb mitral cell layer	Strong		4A
TS28: main olfactory bulb	Strong	Regionally restricted	4A	Distinct fiber staining was detected in the dendrites of mitral and periglomerular cells.
TS28: main olfactory bulb granule cell layer	Weak		4A

Structure	Level	Pattern	Image	Note
TS27: thalamic nucleus	Present	Uniform	4B	Uniform staining was detected in lateral dorsal nucleus, ventroanterior nucleus, and ventrolateral nucleus.
TS27: subthalamic nucleus	Present	Uniform	4B
TS27: zona incerta	Present	Uniform	4B

Structure	Level	Pattern	Image	Note
TS28: CA3 pyramidal cell layer	Strong		4C
TS28: CA1 pyramidal cell layer	Moderate		4C
TS28: CA2 pyramidal cell layer	Moderate		4C
TS28: dentate gyrus granule cell layer	Weak		4C

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Strong		4D

Structure	Level	Pattern	Image	Note
TS28: dorsal motor nucleus of vagus nerve	Present
TS28: edinger-westphal nucleus	Present
TS28: hypoglossal nucleus	Present
TS28: oculomotor nucleus	Present
TS28: pontine nucleus	Present
TS28: red nucleus	Present
TS28: substantia nigra	Present
TS28: trigeminal nucleus	Present
TS28: trochlear nucleus	Present

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5A
TS28: cerebellum external granule cell layer	Present		5A
TS28: cerebellum internal granule cell layer	Moderate		5A	Expression was detected in granule cells and an occasional Golgi (type II) cell.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		5B	Expression increased at P20 compared to P7.
TS28: cerebellum internal granule cell layer	Strong		5B	Expression increased at P20 compared to P7.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Moderate	Regionally restricted	6A	Expression was detected in the interfascicular oligodendrocytes in the corpus callosum. No fiber staining was detected.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Moderate	Regionally restricted	6B	Expression was detected in the interfascicular oligodendrocytes in the deep cerebellar white matter. No fiber staining was detected.

Structure	Level	Pattern	Image	Note
TS25: main olfactory bulb mitral cell layer	Moderate
TS25: main olfactory bulb prospective glomerular layer	Moderate
TS25: main olfactory bulb external plexiform layer	Weak
TS25: cerebral cortex intermediate zone	Strong
TS25: cerebral cortex marginal zone	Strong
TS25: cerebral cortex subventricular zone	Strong
TS25: cortical plate	Strong
TS25: cortical subplate	Strong
TS25: hippocampus	Moderate	Regionally restricted		Expression level was medium in CA1, CA2, and CA3.
TS25: dentate gyrus	Weak
TS25: cerebellum external granule cell layer	Weak
TS25: cerebellum Purkinje cell cluster	Absent

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: main olfactory bulb external plexiform layer	Moderate
TS28: cerebral cortex subventricular zone	Moderate
TS28: cortical layer I	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer IV	Moderate
TS28: cortical layer V	Strong
TS28: cortical layer VI	Strong	Non-Uniform		High expression in cortical layer VIb; medium expression in cortical layer VIa.
TS28: CA1 pyramidal cell layer	Moderate
TS28: CA2 pyramidal cell layer	Moderate
TS28: dentate gyrus	Moderate
TS28: CA3 pyramidal cell layer	Strong
TS28: cerebellum external granule cell layer	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: cerebellum Purkinje cell layer	Moderate

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: main olfactory bulb external plexiform layer	Moderate
TS28: cerebral cortex subventricular zone	Moderate
TS28: cortical layer I	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer IV	Moderate
TS28: cortical layer V	Strong
TS28: cortical layer VI	Strong	Non-Uniform		High expression in cortical layer VIb; medium expression in cortical layer VIa.
TS28: CA1 pyramidal cell layer	Moderate
TS28: CA2 pyramidal cell layer	Moderate
TS28: CA3 pyramidal cell layer	Strong
TS28: dentate gyrus	Weak
TS28: cerebellum external granule cell layer	Moderate
TS28: cerebellum Purkinje cell layer	Strong
TS28: cerebellum internal granule cell layer	Strong

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: main olfactory bulb external plexiform layer	Moderate
TS28: cerebral cortex subventricular zone	Weak
TS28: cortical layer I	Weak
TS28: cortical layer IV	Weak
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer V	Strong
TS28: cortical layer VI	Moderate	Non-Uniform		Medium expression in cortical layer VIb; low expression in cortical layer VIa.
TS28: CA1 pyramidal cell layer	Weak
TS28: CA2 pyramidal cell layer	Weak
TS28: dentate gyrus	Weak
TS28: CA3 pyramidal cell layer	Moderate
TS28: cerebellum Purkinje cell layer	Strong
TS28: cerebellum internal granule cell layer	Strong

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: main olfactory bulb external plexiform layer	Moderate
TS28: cortical layer I	Absent
TS28: cortical layer II	Absent
TS28: cortical layer III	Absent
TS28: cortical layer IV	Absent
TS28: cortical layer V	Moderate
TS28: cortical layer VI	Moderate	Regionally restricted		Medium expression in cortical layer VIb; no expression detected in cortical layer VIa.
TS28: CA1 pyramidal cell layer	Absent
TS28: CA2 pyramidal cell layer	Absent
TS28: CA3 pyramidal cell layer	Absent
TS28: dentate gyrus	Absent
TS28: cerebellum Purkinje cell layer	Strong
TS28: cerebellum internal granule cell layer	Strong