Gene Expression Data
Assay Details
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Assay
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Reference:
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J:133440
Tuy FP, et al., Alternative transcripts of Dclk1 and Dclk2 and their expression in doublecortin knockout mice. Dev Neurosci. 2008;30(1-3):171-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6415558
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Gene symbol:
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Dclk2
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Gene name:
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doublecortin-like kinase 2
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Probe:
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Dclk2 cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Using the exon 16a probe (MGI:6415401) gave the similar expression patterns, but weaker signal.
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Results
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Specimen
6A:
embryonic day 14.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
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Specimen
6B:
embryonic day 14.5
(more )
Specimen
6B:
(close )
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Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
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Specimen
6C:
embryonic day 14.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6D:
embryonic day 14.5
(more )
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: medial ganglionic eminence |
Strong |
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6D
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Expressed throughout the structure, although with a higher density in the subventricular zone and intermediate zone.
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Specimen
6E:
embryonic day 14.5
(more )
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: anterior entopeduncular area |
Strong |
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6E
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Expressed throughout the structure, although with a higher density in the subventricular zone and intermediate zone.
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Specimen
6F:
postnatal day 0
(more )
Specimen
6F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7A:
embryonic day 14.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
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7A
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Specimen
7B:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Mutant Allele(s):
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Dcxtm1.2Ffr/Y
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Age:
|
embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
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7B
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Slight differences in signal in tensity were observed, but considered as an artifact.
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Specimen
7C:
embryonic day 16.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
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7C
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Specimen
7D:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Mutant Allele(s):
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Dcxtm1.2Ffr/Y
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
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7D
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Similar expression as in the wild-type.
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Specimen
7E:
embryonic day 17.5
(more )
Specimen
7E:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: brain |
Present |
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7E
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Specimen
7F:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Mutant Allele(s):
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Dcxtm1.2Ffr/Y
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Age:
|
embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: brain |
Present |
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7F
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Similar expression as in the wild-type.
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Specimen
7G:
embryonic day 16.5
(more )
Specimen
7G:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7H:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Mutant Allele(s):
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Dcxtm1.2Ffr/Y
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7J/K:
embryonic day 16.5
(more )
Specimen
7J/K:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hippocampus |
Strong |
Regionally restricted |
7J/K
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Expressed throughout the developing hippocampal pyramidal cell layer.
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Specimen
7L/M:
embryonic day 16.5; Dcxtm1.2Ffr/Y
(more )
Specimen
7L/M:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Mutant Allele(s):
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Dcxtm1.2Ffr/Y
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Age:
|
embryonic day 16.5
|
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Sex:
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Male
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Type:
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section
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Fixation:
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Paraformaldehyde
|
|
Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hippocampus |
Strong |
Regionally restricted |
7L/M
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Similar expression throughout the developing hippocampal pyramidal cell layer as in the wild-type.
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Specimen
S4A:
embryonic day 14.5
(more )
Specimen
S4A:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
|
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
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S4A
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Specimen
S4B:
embryonic day 14.5; Dcxtm1.2Ffr/Y
(more )
Specimen
S4B:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Mutant Allele(s):
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Dcxtm1.2Ffr/Y
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Age:
|
embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
|
Paraformaldehyde
|
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
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S4B
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Similar expression as in the wild-type.
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Specimen
S4C:
embryonic day 16.5
(more )
Specimen
S4C:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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Age:
|
embryonic day 16.5
|
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
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S4C
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Specimen
S4D:
embryonic day 16.5; Dcxtm1.2Ffr/Y
(more )
Specimen
S4D:
(close )
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Genetic Background:
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either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
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|
Mutant Allele(s):
|
Dcxtm1.2Ffr/Y
|
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Age:
|
embryonic day 16.5
|
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Sex:
|
Male
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Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
|
S4D
|
Similar expression as in the wild-type.
|
Specimen
S4E:
embryonic day 17.5
(more )
Specimen
S4E:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
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Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: brain |
Present |
|
S4E
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|
Specimen
S4F:
embryonic day 17.5; Dcxtm1.2Ffr/Y
(more )
Specimen
S4F:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Mutant Allele(s):
|
Dcxtm1.2Ffr/Y
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: brain |
Present |
|
S4F
|
Similar expression as in the wild-type.
|
Specimen
S4G:
embryonic day 14.5
(more )
Specimen
S4G:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
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Age:
|
embryonic day 14.5
|
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Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S4H:
embryonic day 14.5; Dcxtm1.2Ffr/Y
(more )
Specimen
S4H:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Mutant Allele(s):
|
Dcxtm1.2Ffr/Y
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
|
S4H
|
Similar expression as in the wild-type.
|
Specimen
S4I:
embryonic day 16.5
(more )
Specimen
S4I:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S4J:
embryonic day 16.5; Dcxtm1.2Ffr/Y
(more )
Specimen
S4J:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Mutant Allele(s):
|
Dcxtm1.2Ffr/Y
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cerebral cortex |
Present |
|
S4J
|
Similar expression as in the wild-type.
|
Specimen
S4K/L:
embryonic day 14.5
(more )
Specimen
S4K/L:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
|
S4K/L
|
|
Specimen
S4M/N:
embryonic day 14.5; Dcxtm1.2Ffr/Y
(more )
Specimen
S4M/N:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Mutant Allele(s):
|
Dcxtm1.2Ffr/Y
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
|
S4M/N
|
Similar expression as in the wild-type.
|
Specimen
S5A:
embryonic day 17.5
(more )
Specimen
S5A:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S5B:
embryonic day 17.5; Dcxtm1.2Ffr/Y
(more )
Specimen
S5B:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Mutant Allele(s):
|
Dcxtm1.2Ffr/Y
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S5D/E:
embryonic day 17.5
(more )
Specimen
S5D/E:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hippocampus |
Present |
Regionally restricted |
S5D/E
|
Expressed in the heterotopic CA3 pyramidal layer.
|
Specimen
S5F/G:
embryonic day 17.5; Dcxtm1.2Ffr/Y
(more )
Specimen
S5F/G:
(close )
|
Genetic Background:
|
either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)
|
|
Mutant Allele(s):
|
Dcxtm1.2Ffr/Y
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hippocampus |
Present |
Regionally restricted |
S5F/G
|
Expressed in the heterotopic CA3 pyramidal layer, similar as in the wild-type.
|
|
Analysis Tools