Probe:	DC cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		3A	Less intense signal was observed.
TS22: cerebral cortex ventricular layer	Present		3A	Intensity comparable to the cortical plate.
TS22: ganglionic eminence	Present		3A	Relatively more intense compared to the neocortical labeling.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		3B	Less intense signal was observed, but no major differences compared with the wild-type.
TS22: cerebral cortex ventricular layer	Present		3B	Intensity comparable to the cortical plate, but no major differences compared with the wild-type.
TS22: ganglionic eminence	Present		3B	Relatively more intense compared to the neocortical labeling, but no major differences compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present		3C	Less intense signal was observed.
TS24: cerebral cortex ventricular layer	Present		3C	Intensity comparable to the cortical plate.
TS24: ganglionic eminence	Present		3C	Relatively more intense compared to the neocortical labeling.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present		3D	Less intense signal was observed, but no major differences compared with the wild-type.
TS24: cerebral cortex ventricular layer	Present		3D	Intensity comparable to the cortical plate, but no major differences compared with the wild-type.
TS24: ganglionic eminence	Present		3D	Relatively more intense compared to the neocortical labeling, but no major differences compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Present		3E	Less intense signal was observed.
TS25: cerebral cortex ventricular layer	Present		3E	Intensity comparable to the cortical plate.
TS25: ganglionic eminence	Present		3E	Relatively more intense compared to the neocortical labeling.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Present		3F	Less intense signal was observed, but no major differences compared with the wild-type.
TS25: cerebral cortex ventricular layer	Present		3F	Intensity comparable to the cortical plate, but no major differences compared with the wild-type.
TS25: ganglionic eminence	Present		3F	Relatively more intense compared to the neocortical labeling, but no major differences compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present		3G/H	Less intense signal was observed.
TS24: cerebral cortex ventricular layer	Present		3G/H	Intensity comparable to the cortical plate.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present		3I/J	Less intense signal was observed, but no major differences compared with the wild-type.
TS24: cerebral cortex ventricular layer	Present		3I/J	Intensity comparable to the cortical plate, but no major differences compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	3L/M	Expressed in both the ventricular zone and the pyramidal layer.

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	3N/O	Expressed in both the ventricular zone and the pyramidal layer, but no major differences compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Present		S1G	Less intense signal was observed.
TS25: cerebral cortex ventricular layer	Present		S1G	Intensity comparable to the cortical plate.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Present		S1H	Less intense signal was observed, but no major differences compared with the wild-type.
TS25: cerebral cortex ventricular layer	Present		S1H	Intensity comparable to the cortical plate, but no major differences compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular layer	Present		S1I

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular layer	Present		S1J	No major differences compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS25: hippocampus	Present	Regionally restricted	S1K/L	Expressed in both the ventricular zone and the pyramidal layer, but no major differences compared with the wild-type. Both CA3 pyramidal layers were labeled.