Gene Expression Data
Assay Details
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Assay
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Reference:
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J:255612
Uysal F, et al., DNMT1, DNMT3A and DNMT3B proteins are differently expressed in mouse oocytes and early embryos. J Mol Histol. 2017 Dec;48(5-6):417-426
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6415118
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Gene symbol:
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Dnmt3a
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Gene name:
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DNA methyltransferase 3A
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Antibody:
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anti-Dnmt3a (ab23565)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Relative expression levels are presented in Figs 2B and 4; expression levels fluctuated during the time period examined.
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Results
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Specimen
2A GV:
postnatal
(more  )
Specimen
2A GV:
(close  )
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Genetic Background:
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BALB/c
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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3% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: primary oocyte |
Present |
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2A GV
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germinal vesicle-stage oocyte; expression is strongly localized in the nucleus, weak expression in the cytoplasm
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Specimen
2A MII:
postnatal
(more )
Specimen
2A MII:
(close )
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Genetic Background:
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BALB/c
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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3% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Present |
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2A MII
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metaphase II-stage oocyte; expression is strongly localized in the nucleus, weak expression in the cytoplasm
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Specimen
2A 1C:
embryonic day 0.5
(more )
Specimen
2A 1C:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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3% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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2A 1C
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expression is strongly localized in the nucleus, weak expression in the cytoplasm
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Specimen
2A 2C:
embryonic day 1.0
(more )
Specimen
2A 2C:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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3% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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2A 2C
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expression is strongly localized in the nucleus, weak expression in the cytoplasm
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Specimen
2A 4C:
embryonic day 2.0
(more )
Specimen
2A 4C:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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3% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
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2A 4C
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expression is strongly localized in the nucleus, weak expression in the cytoplasm
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Specimen
2A 8C:
embryonic day 2.5
(more )
Specimen
2A 8C:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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3% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
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2A 8C
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expression is strongly localized in the nucleus, weak expression in the cytoplasm
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Specimen
2A M:
embryonic day 3.0
(more )
Specimen
2A M:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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3% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
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2A M
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expression is strongly localized in the nucleus, weak expression in the cytoplasm
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Specimen
2A B:
embryonic day 3.5
(more )
Specimen
2A B:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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3% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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2A B
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expression is strongly localized in the nucleus, weak expression in the cytoplasm
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Analysis Tools