Gene Expression Data
Assay Details
Assay
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Reference:
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J:64190
Fujita E, et al., Detection of caspase-9 activation in the cell death of the bcl-x-deficient mouse embryo nervous system by cleavage sites-directed antisera. Brain Res Dev Brain Res. 2000 Aug 30;122(2):135-47
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6414784
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Gene symbol:
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Casp9
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Gene name:
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caspase 9
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Antibody:
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anti-m9D353
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
4C:
embryonic day 11.5
(more )
Specimen
4C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: hindbrain |
Present |
Regionally restricted |
4C
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Expression in caudal portions of ventral hindbrain. Rare expressing cells in dorsal hindbrain.
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Specimen
4D:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Bcl2l1tm1Dlo/Bcl2l1tm1Dlo
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: hindbrain |
Present |
Regionally restricted |
4D
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Increased number of expressing cells in caudal portion of ventral hindbrain compared to +/+ embryos.
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Specimen
4I:
embryonic day 11.5
(more )
Specimen
4I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
4J:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Bcl2l1tm1Dlo/Bcl2l1tm1Dlo
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5C:
embryonic day 11.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5D:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Bcl2l1tm1Dlo/Bcl2l1tm1Dlo
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dorsal root ganglion |
Present |
Regionally restricted |
5D
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Increased number of expressing cells compared to +/+ embryos.
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Specimen
5I:
embryonic day 11.5
(more )
Specimen
5I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Trace |
Regionally restricted |
5I
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Rare expressing cells in anterior horn.
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Specimen
5J:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Bcl2l1tm1Dlo/Bcl2l1tm1Dlo
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
5J
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Increased number of expressing cells in anterior horn compared to +/+ embryos.
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Specimen
bcl-x +/+ not shown:
embryonic day 11.5
(more )
Specimen
bcl-x +/+ not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forebrain |
Trace |
Regionally restricted |
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Rare expressing cells in forebrain, dorsal midbrain, and dorsal spinal cord.
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TS19: future spinal cord |
Trace |
Regionally restricted |
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Rare expressing cells in forebrain, dorsal midbrain, and dorsal spinal cord.
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TS19: midbrain |
Trace |
Regionally restricted |
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Rare expressing cells in forebrain, dorsal midbrain, and dorsal spinal cord.
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Specimen
bcl-x -/- not shown:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Bcl2l1tm1Dlo/Bcl2l1tm1Dlo
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
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Increased number of expressing cells in rostral portions of ventral hindbrain, ventral midbrain and ventral spinal cord.
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TS19: hindbrain |
Present |
Regionally restricted |
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Increased number of expressing cells in rostral portions of ventral hindbrain, ventral midbrain and ventral spinal cord.
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TS19: midbrain |
Present |
Regionally restricted |
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Increased number of expressing cells in rostral portions of ventral hindbrain, ventral midbrain and ventral spinal cord.
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