About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:138147 Gros-Louis F, et al., Als2 mRNA splicing variants detected in KO mice rescue severe motor dysfunction phenotype in Als2 knock-down zebrafish. Hum Mol Genet. 2008 Sep 1;17(17):2691-702
Assay type: Western blot
MGI Accession ID: MGI:6414778
Gene symbol: Nefh
Gene name: neurofilament, heavy polypeptide
Antibody: SMI 32
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The antibody recognizes the nonphosporylated epitope in neurofilament H.
Results Image: 3C SMI-32
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
+/+ P m 7-22 TS28: sciatic nerve Present C57BL/6 Not Specified
+/- P m 7-22 TS28: sciatic nerve Present either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Als2tm1Garo/Als2+Not Specified
-/- P m 7-22 TS28: sciatic nerve Present (a) either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Als2tm1Garo/Als2tm1GaroNot Specified
+/+ P m 7-22 TS28: spinal cord Present C57BL/6 Not Specified
+/- P m 7-22 TS28: spinal cord Present either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Als2tm1Garo/Als2+Not Specified
-/- P m 7-22 TS28: spinal cord Present either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Als2tm1Garo/Als2tm1GaroNot Specified
+/+ P m 7-22 TS28: cerebral cortex Present C57BL/6 Not Specified
+/- P m 7-22 TS28: cerebral cortex Present either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Als2tm1Garo/Als2+Not Specified
-/- P m 7-22 TS28: cerebral cortex Present (a) either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Als2tm1Garo/Als2tm1GaroNot Specified
+/+ P m 7-22 TS28: cerebellum Present C57BL/6 Not Specified
+/- P m 7-22 TS28: cerebellum Present either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Als2tm1Garo/Als2+Not Specified
-/- P m 7-22 TS28: cerebellum Weak either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Als2tm1Garo/Als2tm1GaroNot Specified
Notes:
(a) There is a slight increase in expression in this mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory