Probe:	mPlexa1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Strong		1A
TS17: neural tube	Strong	Regionally restricted	1A	Higher levels of expression are observed in the ventral neural tube (the ventral rim).
TS17: forebrain	Present	Widespread	1A
TS17: hindbrain	Present	Widespread	1A
TS17: midbrain	Present	Widespread	1A

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Non-Uniform	1B	Strongly expressing cells are found in a medially positioned line in the lateral hindbrain, extending more caudally into the medulla oblongata.
TS20: medulla oblongata	Strong	Non-Uniform	1B	Strongly expressing cells are found in a medially positioned line in the lateral hindbrain, extending more caudally into the medulla oblongata.
TS20: forebrain	Present		1B
TS20: midbrain	Present		1B
TS20: spinal cord	Present		1B

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Non-Uniform	1C	Strongly expressing cells are observed near the floorplate.
TS20: forebrain	Present		1C
TS20: midbrain	Present		1C

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Non-Uniform	1D	Higher levels of expression are seen in cells abutting the facial nucleus.
TS22: cerebellum	Present		1D
TS22: hypothalamus	Present		1D
TS22: medulla oblongata	Present		1D
TS22: thalamus	Present		1D

Structure	Level	Pattern	Image	Note
TS24: hindbrain	Strong	Non-Uniform	1E	Strongly expressing cells are seen ventrally.
TS24: facial nucleus	Present		1E	A higher magnification is shown in the inlay.