Antibody:	CA-RP X antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Specimens were post-fixed in 10% formalin.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		4a	Expression is in neural cell bodies.

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Present		4b	Expression is in neural cell bodies and in axon and myelin sheath.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	5a	Expression in this mutant is in the neural cell bodies and is virtually absent in the myelin sheath.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present		5b	Expression is in neural cell bodies and in axon and myelin sheath

Structure	Level	Pattern	Image	Note
TS28: brainstem	Moderate	Regionally restricted		Expression is in neural cells and is absent in glial cells.
TS28: midbrain	Moderate	Regionally restricted		Expression is in neural cells and is absent in glial cells.
TS28: telencephalon	Moderate	Regionally restricted		Expression is in neural cells and is absent in glial cells.
TS28: cerebellum Purkinje cell layer	Moderate
TS28: dentate nucleus	Moderate
TS28: hippocampus	Moderate
TS28: brain ependyma	Weak
TS28: cerebellum molecular layer	Weak
TS28: choroid plexus	Weak
TS28: cerebellum granule cell layer	Absent

Structure	Level	Pattern	Image	Note
TS16: future brain	Present	Regionally restricted		Expression is in the neuroprogenitor cells in the subventricular area.
TS16: heart	Present
TS16: surface ectoderm	Present

Structure	Level	Pattern	Image	Note
TS24: brain	Present	Regionally restricted		Expression is in neural cells migrating to the cortex.
TS24: heart	Present
TS24: skin	Present