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Gene Expression Data
Assay Details
Assay
Reference: J:285737 Cui W, et al., MCRS1 is essential for epiblast development during early mouse embryogenesis. Reproduction. 2020 Jan;159(1):1-13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6404983
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog antibody (ab80892)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen +/+: embryonic day 3.5 (more )
Note: Triple labeled: red - Nanog; white - Cdx2 (assay MGI:6404960); green - Sox17 (assay MGI:6404981). Blastocysts were flushed out at E3.5, then fixed directly for staining.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4_Nanog Mcrs1+/+ , S4_Merge Mcrs1+/+ Expressed in epiblast.
Specimen +/-: embryonic day 3.5; Mcrs1tm1.1(KOMP)Vlcg/Mcrs1+ (more )
Note: Triple labeled: red - Nanog; white - Cdx2 (assay MGI:6404960); green - Sox17 (assay MGI:6404981). Blastocysts were flushed out at E3.5, then fixed directly for staining.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4_Merge Mcrs1+/- , S4_Nanog Mcrs1+/- Similar as in the wild-type. Expressed in epiblast.
Specimen -/-: embryonic day 3.5; Mcrs1tm1.1(KOMP)Vlcg/Mcrs1tm1.1(KOMP)Vlcg (more )
Note: Triple labeled: red - Nanog; white - Cdx2 (assay MGI:6404960); green - Sox17 (assay MGI:6404981). Blastocysts were flushed out at E3.5, then fixed directly for staining.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4_Nanog Mcrs1-/- , S4_Merge Mcrs1-/- Similar as in the wild-type. Expressed in epiblast.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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